Search

Your search keyword '"Traugott, Michael"' showing total 2 results
Start Over Author "Traugott, Michael" Topic arion lusitanicus
2 results on '"Traugott, Michael"'

1. A multiplex PCR assay for detecting slug species common in European arable land in the diet of carabid beetles.

Author

Guenay-Greunke, Yasemin, Bohan, David A., Traugott, Michael, and Wallinger, Corinna

Subjects
CYTOCHROME oxidase, GROUND beetles, ARABLE land, RIBOSOMAL RNA, BIOLOGICAL pest control agents, POLYMERASE chain reaction
Abstract

DNA-based diet analysis of natural enemies is a valuable tool for unravelling the food choice of predators in agroecosystems. It enables the rapid identification of potential biocontrol agents of invertebrate pests. Here, we present a new multiplex PCR system for the identification of pest slug species in the diet of their natural enemies such as carabid beetles. It comprises three species-specific primers targeting the mitochondrial cytochrome c oxidase subunit I (COI) gene to detect DNA of the common garden slug, Arion distinctus (Stylommatophora: Arionidae), the Iberian slug, Arion lusitanicus (Stylommatophora: Arionidae) and the grey field slug Deroceras reticulatum (Stylommatophora: Agriolimacidae). We also include (super)family-specific primers for Arionidae and Limacoidea, which amplify parts of the 28S gene for ribosomal RNA (rRNA) in order to identify a wider range of slugs. The amplicons for Arionidae can be assigned to a total of seven Central European slug species of this family and the amplicons for Limacoidea to ten species. The multiplex assay showed high specificity against DNA extracts of field-collected slugs and co-occurring invertebrates. The assay also exhibited high sensitivity, which was confirmed by testing it with 223 dietary samples from field-collected carabids as potential natural enemies of slugs in agroecosystems. This methodology represents a new, cost-effective, highly sensitive and specific approach for the identification of common Central European slug species as well as for analysing trophic interactions to identify natural enemies for further biological control development. It can also be applied in any study where a rapid and reliable identification of slugs is needed. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

2. Feeding activity and survival of slug Arion lusitanicus (Gastropoda: Arionidae) exposed to the rhabditid nematode, Phasmarhabditis hermaphrodita (Nematoda: Rhabditidae)

Author

Grubišić, Dinka, Hamel, Tina, Gotlin Čuljak, Tanja, Loparić, Ana, Brmež, Mirjana, and Jehle, Johannes A., Bažok, Renata, Crickmore, Neil, Lopez-Ferber, Miguel, Glazer, Itamar, Quesada-Moraga, Enrique, Traugott, Michael.

Subjects
fungi, Arion lusitanicus, molluscicides, parasitic nematodes, Phasmarhabditis hermaphrodita, slugs, slug control
Abstract

Slugs are important pests of cultivated plants in Croatia. In many sites slug species Arion lusitanicus (Mabille, 1868) (Gastropoda: Arionidae) has become the most frequent species, which is very hard to control by chemical molluscicides. Since 1996 a biological molluscicide based on nematode Phasmarhabditis hermaphrodita (Schneider, 1859) (Nematoda: Rhabditidae) has been formulated as an effective product for slug control. In order to establish feeding activity and survival of adult A. lusitanicus specimens exposed to parasitic nematode P. hermaphrodita and to compare its efficiency to efficiency of chemical molluscicides, a laboratory experiment was performed. Adult specimens of A. lusitanicus (feeded on lettuce leaves in flower pots) were exposed to P. hermaphrodita, metaldehyde and methiocarb treatments. Food consumption of slug specimens was measured daily. Survival of slugs was observed to their death, up to 30th day. In the first week of investigation, chemical molluscicide treatments were found to differ significantly from the biological product and control. At the treatments treated by nematodes, daily leaf area consumption was also reduced and was significantly different from the control treatment. Food consumption was reduced on both chemical and biological treatments but the most of adult speciments of A. lusitanicus survived and continued to feed. To the end of the second week of investigation, food consumption decreased on all treatments and was mainly uniform with no significant differences between treatments. During the experiment, the slugs were dying within the period of 3 to 30 days at the treatments treated by nematode P. hermaphrodita or in the period of 9 to 24 days at the treatments treated by metaldehyde and methiocarb. Because the tolerance level to slug damages in lettuce market is effectively zero, these results indicate a failure of biological product based on P. hermaphrodita in control of adult specimens of A. lusitanicus as well as a failure of chemical molluscicides. These data point at a great need for integrated control of slug damage which must include cultural and different alternative control measures, not only chemical control.

Published
2013

Catalog

Books, media, physical & digital resources
See catalog results