1. Prenylated Coumaric Acids from Artemisia scoparia Beneficially Modulate Adipogenesis.
- Author
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Ribnicky D, Beom Kim S, Poulev A, Wang Y, Boudreau A, Raskin I, Bisson J, Ray GJ, Chen SN, Richard A, Stephens JM, and Pauli GF
- Subjects
- 3T3-L1 Cells, Adiponectin metabolism, Animals, Coumaric Acids isolation & purification, Lipolysis drug effects, Mice, Phytochemicals isolation & purification, Phytochemicals pharmacology, Prenylation, Tumor Necrosis Factor-alpha metabolism, Adipocytes drug effects, Adipogenesis drug effects, Artemisia chemistry, Coumaric Acids pharmacology
- Abstract
Two new diprenylated coumaric acid isomers ( 1a and 1b ) and two known congeners, capillartemisin A ( 2 ) and B ( 3 ), were isolated from Artemisia scoparia as bioactive markers using bioactivity-guided HPLC fractionation. Their structures were determined by spectroscopic means, including 1D and 2D NMR methods and LC-MS, with their purity assessed by 1D
1 H pure shift qNMR spectroscopic analysis. The bioactivity of compounds was evaluated by enhanced accumulation of lipids, as measured using Oil Red O staining, and by increased expression of several adipocyte marker genes, including adiponectin in 3T3-L1 adipocytes relative to untreated negative controls. Compared to the plant's 80% EtOH extract, these purified compounds showed significant but still weaker inhibition of TNFα-induced lipolysis in 3T3-L1 adipocytes. This suggests that additional bioactive substances are responsible for the multiple metabolically favorable effects on adipocytes observed with Artemisia scoparia extract.- Published
- 2021
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