1. Mimics and artefacts of measurable residual disease in a highly sensitive multicolour flow cytometry assay for B-lymphoblastic leukaemia/lymphoma: critical consideration for analysis of measurable residual disease
- Author
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Niharika Dasgupta, Chetan Dhamne, Nikhil Patkar, Shripad Banavali, Nilesh Deshpande, Dhanalaxmi Shetty, Papagudi Ganesan Subramanian, Gaurav Narula, Harshini Sriram, Nirmalya Roy Moulik, Sitaram Ghogale, Karishma Girase, Sweta Rajpal, Prashant Tembhare, Gaurav Chatterjee, Gauri Arolkar, Twinkle Khanka, Shefali Verma, and Sumeet Gujral
- Subjects
Stromal cell ,Neoplasm, Residual ,Clinical Decision-Making ,CD34 ,Sensitivity and Specificity ,CD19 ,Flow cytometry ,Immunophenotyping ,hemic and lymphatic diseases ,Precursor B-Cell Lymphoblastic Leukemia-Lymphoma ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Biomarkers, Tumor ,Humans ,biology ,medicine.diagnostic_test ,Chemistry ,Mesenchymal stem cell ,Disease Management ,Reproducibility of Results ,Hematology ,Induction Chemotherapy ,Flow Cytometry ,Prognosis ,Treatment Outcome ,Cancer research ,biology.protein ,Interleukin-3 receptor ,Stem cell ,Artifacts - Abstract
High-sensitivity multicolour flow cytometry (MFC)-based B-lymphoblastic leukaemia (B-ALL) measurable residual disease (BMRD) assay is increasingly being used in clinical practice. Herein, we describe six consistently present low-level populations immunophenotypically mimicking abnormal B-ALL blasts in 441 BMRD samples from 301 children. These included CD19+ CD123+ plasmacytoid dendritic cells differentiating from lymphoid precursors, CD10+ transitional B cells with CD10+ /CD38dim-to-negative/CD20bright/CD45bright phenotype, CD19+ natural killer (NK) cells, CD73bright/CD10+ mesenchymal stromal/stem cells, CD73bright/CD34+ endothelial cells, and a CD34+ CD38dim-to-negative/CD10- /CD20bright/CD45bright subset of mature B cells. We provide the proportions, comprehensive immunophenotype, and practical clues for proper identification of these low-level populations. Knowledge regarding the presence and immunophenotype of these mimics is essential for accurate interpretation in high-sensitivity MFC-BMRD analysis.
- Published
- 2021