1. A comparative genomics study of 23 Aspergillus species from section Flavi.
- Author
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Kjærbølling I, Vesth T, Frisvad JC, Nybo JL, Theobald S, Kildgaard S, Petersen TI, Kuo A, Sato A, Lyhne EK, Kogle ME, Wiebenga A, Kun RS, Lubbers RJM, Mäkelä MR, Barry K, Chovatia M, Clum A, Daum C, Haridas S, He G, LaButti K, Lipzen A, Mondo S, Pangilinan J, Riley R, Salamov A, Simmons BA, Magnuson JK, Henrissat B, Mortensen UH, Larsen TO, de Vries RP, Grigoriev IV, Machida M, Baker SE, and Andersen MR
- Subjects
- Aspergillus flavus classification, Aspergillus flavus enzymology, Aspergillus oryzae classification, Aspergillus oryzae enzymology, Bioreactors, Carbohydrate Metabolism genetics, Crops, Agricultural microbiology, DNA, Fungal genetics, Fermentation, Fermented Foods, Fungal Proteins genetics, Fungal Proteins metabolism, Metabolic Networks and Pathways genetics, Multigene Family, Phenotype, Phylogeny, Plant Diseases prevention & control, Secondary Metabolism genetics, Aspergillus flavus genetics, Aspergillus oryzae genetics, Genome, Fungal genetics, Genomics
- Abstract
Section Flavi encompasses both harmful and beneficial Aspergillus species, such as Aspergillus oryzae, used in food fermentation and enzyme production, and Aspergillus flavus, food spoiler and mycotoxin producer. Here, we sequence 19 genomes spanning section Flavi and compare 31 fungal genomes including 23 Flavi species. We reassess their phylogenetic relationships and show that the closest relative of A. oryzae is not A. flavus, but A. minisclerotigenes or A. aflatoxiformans and identify high genome diversity, especially in sub-telomeric regions. We predict abundant CAZymes (598 per species) and prolific secondary metabolite gene clusters (73 per species) in section Flavi. However, the observed phenotypes (growth characteristics, polysaccharide degradation) do not necessarily correlate with inferences made from the predicted CAZyme content. Our work, including genomic analyses, phenotypic assays, and identification of secondary metabolites, highlights the genetic and metabolic diversity within section Flavi.
- Published
- 2020
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