Huang, Liming, Ding, Lijian, Li, Xiaohui, Wang, Ning, Yan, Yushan, Yang, Mengxiang, Cui, Wei, Benjamin Naman, C., Cheng, Kejun, Zhang, Weiyan, Zhang, Bin, Jin, Haixiao, and He, Shan
• Two new lactones (1 and 2) and two known (3 and 4) were discovered from a culture of the marine sponge-associated fungus. • The structures were characterized by comprehensive spectroscopic methods. • Compounds 1 and 4 exhibited significant inhibition against the lateral root growth of Arabidopsis thaliana Columbia-0. • Compound 4 showed moderate inhibitory effects on CCRF-CEM and K562 cancer cell lines. Two new γ -lactones, aspergilactones A (1) and B (2), were discovered along with two known compounds, annularin A (3) and pericoterpenoid A (4), from a culture of the sponge-associated fungus Aspergillus sp. LS45. The planar structures of 1 – 4 were characterized using comprehensive spectroscopic methods and comparison with literature data. The absolute configurations of 1 and 2 were determined by comparison of electronic circular dichroism (ECD) spectroscopic and optical rotation data with those of known analogues as well as calculated ECD analysis. Compounds 1 – 4 were tested in a variety of bioassays, and both 1 and 4 exhibited significant inhibition against the lateral root growth of Arabidopsis thaliana Columbia-0 at a concentration of 100 μM. In addition, the in vitro cytotoxic activities of 1 – 4 against six human cancer cell lines CCRF-CEM, K562, BGC823, AGS, HCT-116 and MDA-MB-231 were evaluated. Compound 4 showed moderate inhibitory effects on CCRF-CEM and K562 cancer cell lines with IC 50 values of 13.8 ± 1.6 and 12.9 ± 2.5 μM, respectively. However, compounds 1 – 4 did not show any notable AChE inhibitory activity in vitro. [ABSTRACT FROM AUTHOR]