1. Aspirin increases apolipoprotein-A-I-mediated cholesterol efflux via enhancing expression of ATP-binding cassette transporter A1.
- Author
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Wang YH, Chen YF, Chen SR, Chen X, Chen JW, Shen XY, Mou YG, and Liu PQ
- Subjects
- ATP Binding Cassette Transporter 1, Animals, Blotting, Western, Cells, Cultured, Gene Expression Regulation drug effects, Mice, PPAR alpha metabolism, Platelet Aggregation Inhibitors pharmacology, RNA, Small Interfering administration & dosage, Reverse Transcriptase Polymerase Chain Reaction, Scintillation Counting, Transfection, ATP-Binding Cassette Transporters genetics, Apolipoprotein A-I metabolism, Aspirin pharmacology, Cholesterol metabolism
- Abstract
Background: The efflux of cellular cholesterol mediated by apolipoprotein (apo)A-I and ATP-binding cassette transporter A1 (ABCA1) is a major pathway of reverse cholesterol transport. We investigated the effect of aspirin on this process., Methods: The expression levels of ABCA1 in RAW264.7 cells were determined using reverse transcription polymerase chain reaction and Western blot analysis. ³H-cholesterol efflux was measured by scintillation counting., Results: 0.5 mmol/l aspirin increased apoA-I-mediated cholesterol efflux and increased the expression of ABCA1. By increasing the dose of aspirin higher than 0.5 mmol/l, ABCA1 expression and function were significantly decreased. In cells transfected with a specific peroxisome proliferator-activated receptor (PPAR)-α small interfering RNA, the induction of ABCA1 expression and apoA-I-mediated ³H-cholesterol efflux by aspirin were substantially suppressed., Conclusions: The data demonstrate that low-dose aspirin increases ABCA1 expression via a PPAR-α-dependent mechanism and increases apoA-I-mediated cholesterol efflux., (Copyright © 2010 S. Karger AG, Basel.)
- Published
- 2010
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