1. Role of Matrix Metalloproteinase-2 in Eosinophil-Mediated Airway Remodeling.
- Author
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Kuwabara Y, Kobayashi T, D'Alessandro-Gabazza CN, Toda M, Yasuma T, Nishihama K, Takeshita A, Fujimoto H, Nagao M, Fujisawa T, and Gabazza EC
- Subjects
- Adoptive Transfer, Airway Remodeling drug effects, Animals, Asthma pathology, Cell Culture Techniques, Cell Differentiation immunology, Cell Line, Cell Movement drug effects, Cell Movement immunology, Coculture Techniques, Eosinophils metabolism, Eosinophils transplantation, Female, Humans, Matrix Metalloproteinase 2 genetics, Matrix Metalloproteinase 2 immunology, Matrix Metalloproteinase Inhibitors pharmacology, Mice, Mice, Inbred C57BL, Mice, Transgenic, Airway Remodeling immunology, Asthma immunology, Eosinophils immunology, Matrix Metalloproteinase 2 metabolism, Myofibroblasts physiology
- Abstract
Airway remodeling is responsible for the progressive decline of lung function in bronchial asthma. Matrix metalloproteinase-2 and fibroblast-to-myofibroblast transition are involved in tissue remodeling. Here we evaluated whether eosinophils play a role in fibroblasts-to-myofibroblasts transition and in the expression of matrix metalloproteinase-2. We co-cultured human eosinophils with human fetal lung fibroblast-1 cells, assessed the expression of remodeling-associated molecules by immunoassays and polymerase-chain reaction, and eosinophils-mediated migration of human fetal lung fibroblast-1 cells using a Boyden chamber. To clarify the participation of matrix metalloproteinase-2 in airway remodeling we administered bone marrow-derived eosinophils by intra-tracheal route to transgenic mice overexpressing the human matrix metalloproteinase-2. The expression of α-smooth muscle actin significantly increased in human fetal lung fibroblast-1 cells co-cultured with human eosinophils compared to controls. There was enhanced expression of matrix metalloproteinase-2 during fibroblast-to-myofibroblast transition. An inhibitor of matrix metalloproteinases blocked eosinophils-associated fibroblast-to-myofibroblast transition and increased migration of fibroblasts. The human matrix metalloproteinase-2 transgenic mice receiving adoptive transfer of mouse eosinophils exhibited increased inflammation and advanced airway remodeling compared to wild type mice. This study demonstrated that eosinophils induce fibroblast-to-myofibroblast transition, secretion of matrix metalloproteinase-2, accelerated migration of fibroblasts, and promote matrix metalloproteinase-2-related airway remodeling. These findings provide a novel mechanistic pathway for eosinophil-associated airway remodeling in bronchial asthma.
- Published
- 2018
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