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4 results on '"Flavie Vigier"'

1. Photochemistry on the Space Station—Antibody Resistance to Space Conditions after Exposure Outside the International Space Station

Author

Bartos Przybyla, Michel Dobrijevic, Mickael Baqué, Didier Chaput, Gaëlle Coussot, Clément Faye, Thomas Berger, Flavie Vigier, O. Vandenabeele-Trambouze, Aurélie Le Postollec, Hervé Cottin, Sebastien Incerti, ASP 2019, Laboratoire d'Astrophysique de Bordeaux [Pessac] (LAB), Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 2 - Sciences et Techniques (UM2), Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Brest (UBO), Centre d'Etudes Nucléaires de Bordeaux Gradignan (CENBG), Université Sciences et Technologies - Bordeaux 1-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Centre National d'Études Spatiales [Toulouse] (CNES), Laboratoire Interuniversitaire des Systèmes Atmosphériques (LISA (UMR_7583)), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut national des sciences de l'Univers (INSU - CNRS), and Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)

Subjects
010504 meteorology & atmospheric sciences, Extraterrestrial Environment, Photochemistry, education, Space (mathematics), 01 natural sciences, Antibodies, Planetary exploration, 0103 physical sciences, International Space Station, Spacecraft, 010303 astronomy & astrophysics, Cosmic rays, Antibody, Horseradish Peroxidase, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, Remote sensing, [PHYS]Physics [physics], [SDU.ASTR]Sciences of the Universe [physics]/Astrophysics [astro-ph], Astrobiology, Agricultural and Biological Sciences (miscellaneous), Biochip, Space and Planetary Science, Environmental science, Protein Binding
Abstract

International audience; Antibody-based analytical instruments are under development to detect signatures of life on planetary bodies.Antibodies are molecular recognition reagents able to detect their target at sub-nanomolar concentrations, withhigh affinity and specificity. Studying antibody binding performances under space conditions is mandatory toconvince space agencies of the adequacy of this promising tool for planetary exploration.To complement previous ground-based experiments on antibody resistance to simulated irradiation, weevaluate in this paper the effects of antibody exposure to real space conditions during the EXPOSE-R2 missionoutside the International Space Station. The absorbed dose of ionizing radiation recorded during the 588 days ofthis mission (220 mGy) corresponded to the absorbed dose expected during a mission to Mars. Moreover,samples faced, at the same time as irradiation, thermal cycles, launch constraints, and long-term storage. Amodel biochip was used in this study with antibodies in freeze-dried form and under two formats: free orcovalently grafted to a solid surface.We found that antibody-binding performances were not significantly affected by cosmic radiation, and morethan 40% of the exposed antibody, independent of its format, was still functional during all this experiment. Weconclude that antibody-based instruments are well suited for in situ analysis on planetary bodies. Key Words:Astrobiology—Cosmic rays—Biochip—Antibody—Planetary exploration. Astrobiology 19, 1053–1062.

Published
2019
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2. Photochemistry on the Space Station—Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station

Author

Jerome Caron, Didier Chaput, Gaëlle Coussot, Bartos Przybyla, Corinne Ravelet, Clément Faye, Michel Dobrijevic, Thomas Berger, Sebastien Incerti, Emmanuelle Fiore, Flavie Vigier, O. Vandenabeele-Trambouze, Aurélie Le Postollec, Mickael Baqué, Hervé Cottin, Eric Peyrin, Université Montpellier 2 - Sciences et Techniques (UM2), Laboratoire d'Astrophysique de Bordeaux [Pessac] (LAB), Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre d'Etudes Nucléaires de Bordeaux Gradignan (CENBG), Université Sciences et Technologies - Bordeaux 1-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Université de Brest (UBO), Laboratoire Interuniversitaire des Systèmes Atmosphériques (LISA (UMR_7583)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut national des sciences de l'Univers (INSU - CNRS), Université Grenoble Alpes (UGA), Centre National d'Études Spatiales [Toulouse] (CNES), ASP 2019, Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), and Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Extraterrestrial Environment, 010504 meteorology & atmospheric sciences, Photochemistry, Ultraviolet Rays, Aptamer, education, Cosmic ray, Radiation, 01 natural sciences, Signal, Aptamers, Ion, Optics, 0103 physical sciences, International Space Station, Irradiation, Spacecraft, 010303 astronomy & astrophysics, Cosmic rays, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, Physics, [PHYS]Physics [physics], [SDU.ASTR]Sciences of the Universe [physics]/Astrophysics [astro-ph], business.industry, Temperature, Mars Exploration Program, Aptamers, Nucleotide, Astrobiology, Agricultural and Biological Sciences (miscellaneous), Biochip, Space and Planetary Science, business
Abstract

International audience; Some microarray-based instruments that use bioaffinity receptors such as antibodies or aptamers are under development to detect signatures of past or present life on planetary bodies. Studying the resistance of such instruments against space constraints and cosmic rays in particular is a prerequisite. We used several ground-based facilities to study the resistance of aptamers to various types of particles (protons, electrons, neutrons, and carbon ions) at different energies and fluences. We also tested the resistance of aptamers during the EXPOSE-R2 mission outside the International Space Station (ISS). The accumulated dose measured after the 588 days of this mission (220 mGy) corresponds to the accumulated dose that can be expected during a mission to Mars. We found that the recognition ability of fluorescently labeled aptamers was not significantly affected during short-term exposure experiments taking into account only one type of radiation at a time. However, we demonstrated that the same fluorescent dye was significantly affected by temperature variations (−21°C to +58°C) and storage throughout the entirety of the ISS experiment (60% of signal loss). This induced a large variability of aptamer signal in our analysis. However, we found that >50% of aptamers were still functional after the whole EXPOSE-R2 mission. We conclude that aptamer-based instruments are well suited for in situ analysis on planetary bodies, but the detection step requires additional investigations.

Published
2019
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3. Biochip-based instruments development for space exploration: influence of the antibody immobilization process on the biochip resistance to freeze-drying, temperature shifts and cosmic radiations

Author

A. Le Postollec, Michel Dobrijevic, Gaëlle Coussot, Clément Faye, Flavie Vigier, O. Vandenabeele-Trambouze, Sebastien Incerti, Thomas Moreau, Mickael Baqué, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), COLCOM, Cap Alpha, DLR Institute of Planetary Research, German Aerospace Center (DLR), Laboratoire d'Astrophysique de Bordeaux [Pessac] (LAB), Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre d'Etudes Nucléaires de Bordeaux Gradignan (CENBG), Université Sciences et Technologies - Bordeaux 1-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), ASP 2017, Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de microbiologie des environnements extrêmophiles (LM2E), Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), and Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)

Subjects
010504 meteorology & atmospheric sciences, Physics and Astronomy (miscellaneous), search for Extraterrestrial Life, [SDU.ASTR.EP]Sciences of the Universe [physics]/Astrophysics [astro-ph]/Earth and Planetary Astrophysics [astro-ph.EP], Analytical chemistry, biochip, chemistry.chemical_element, 01 natural sciences, Fluence, Ion, Freeze-drying, Dendrimer, 0103 physical sciences, Earth and Planetary Sciences (miscellaneous), Irradiation, Biochip, 010303 astronomy & astrophysics, Ecology, Evolution, Behavior and Systematics, 0105 earth and related environmental sciences, space constraints, Astrobiology, chemistry, 13. Climate action, Space and Planetary Science, Covalent bond, Carbon
Abstract

Due to the diversity of antibody (Ab)-based biochips chemistries available and the little knowledge about biochips resistance to space constraints, immobilization of Abs on the surface of the biochips dedicated to Solar System exploration is challenging. In the present paper, we have developed ten different biochip models including covalent or affinity immobilization with full-length Abs or Ab fragments. Ab immobilizations were carried out in oriented/non-oriented manner using commercial activated surfaces withN-hydroxysuccinic ester (NHS-surfaces) or homemade surfaces using three generations of dendrimers (dendrigraft of poly L-lysine (DGL) surfaces). The performances of the Ab -based surfaces were cross-compared on the following criteria: (i) analytical performances (expressed by both the surface density of immobilized Abs and the amount of antigens initially captured by the surface) and (ii) resistance of surfaces to preparation procedure (freeze-drying, storage) or spatial constraints (irradiation and temperature shifts) encountered during a space mission. The latter results have been expressed as percentage of surface binding capacity losses (or percentage of remaining active Abs). The highest amount of captured antigen was achieved with Ab surfaces having full-length Abs and DGL-surfaces that have much higher surface densities than commercial NHS-surface. After freeze-drying process, thermal shift and storage sample exposition, we found that more than 80% of surface binding sites remained active in this case. In addition, the resistance of Ab surfaces to irradiation with particles such as electron, carbon ions or protons depends not only on the chemistries (covalent/affinity linkages) and strategies (oriented/non-oriented) used to construct the biochip, but also on the type, energy and fluence of incident particles. Our results clearly indicate that full-length Ab immobilization on NHS-surfaces and DGL-surfaces should be preferred for potential use in instruments for planetary exploration.

Published
2017
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4. Irradiation effects on antibody performance in the frame of biochip-based instruments development for space exploration

Author

Michel Dobrijevic, Clément Faye, A. Le Postollec, Odile Vandenabeele-Trambouze, Jerome Caron, Flavie Vigier, Gaëlle Coussot, Thibault Moreau, Mickael Baqué, Sebastien Incerti, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), ASP 2017, Laboratoire d'Astrophysique de Bordeaux [Pessac] (LAB), Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Génétique, Reproduction et Développement (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), COLCOM, Cap Alpha, Centre d'Etudes Nucléaires de Bordeaux Gradignan (CENBG), Université Sciences et Technologies - Bordeaux 1-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut Bergonié [Bordeaux], UNICANCER, Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), and Université Sciences et Technologies - Bordeaux 1 (UB)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)

Subjects
010504 meteorology & atmospheric sciences, Physics and Astronomy (miscellaneous), Proton, [SDU.ASTR.EP]Sciences of the Universe [physics]/Astrophysics [astro-ph]/Earth and Planetary Astrophysics [astro-ph.EP], astrobiology, biochip, Cosmic ray, Electron, Radiation, 01 natural sciences, Fluence, Nuclear physics, Optics, cosmic rays, 0103 physical sciences, Earth and Planetary Sciences (miscellaneous), Neutron, Irradiation, 010303 astronomy & astrophysics, Ecology, Evolution, Behavior and Systematics, 0105 earth and related environmental sciences, Physics, business.industry, Mars Exploration Program, Space and Planetary Science, search for extraterrestrial life, business
Abstract

Several instruments based on immunoassay techniques have been proposed for life-detection experiments in the framework of planetary exploration but few experiments have been conducted so far to test the resistance of antibodies against cosmic ray particles. We present several irradiation experiments carried out on both grafted and free antibodies for different types of incident particles (protons, neutrons, electrons and 12C) at different energies (between 9 MeV and 50 MeV) and different fluences. No loss of antibodies activity was detected for the whole set of experiments except when considering protons with energy between 20 and 30 MeV (on free and grafted antibodies) and fluences much greater than expected for a typical planetary mission to Mars for instance. Our results on grafted antibodies suggest that biochip-based instruments must be carefully designed according to the expected radiation environment for a given mission. In particular, a surface density of antibodies much larger than the expected proton fluence would prevent significant loss of antibodies activity and thus assuring a successful detection.

Published
2017
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