1. Molecular and Functional Properties of Regional Astrocytes in the Adult Brain.
- Author
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Morel, Lydie, Chiang, Ming Sum R., Haruki Higashimori, Shoneye, Temitope, Iyer, Lakshmanan K., Yelick, Julia, Tai, Albert, and Yongjie Yang
- Subjects
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ASTROCYTES , *CENTRAL nervous system , *RIBOSOMES , *RNA sequencing , *MESSENGER RNA , *CEREBRAL cortex , *HIPPOCAMPUS (Brain) - Abstract
The molecular signature and functional properties of astroglial subtypes in the adult CNS remain largely undefined. By using translational ribosome affinity purification followed by RNA-Seq, we profiled astroglial ribosome-associated (presumably translating) mRNAs in major cortical and subcortical brain regions (cortex, hippocampus, caudate-putamen, nucleus accumbens, thalamus, and hypothalamus) of BACfl/d/tf/l-translational ribosome affinity purification (TRAP) mice (both sexes). We found that the expression of astroglial translating mRNAs closely follows the dorsoventral axis, especially from cortex/hippocampus to thalamus/hypothalamus posteriorly. This region-specific expression pattern of genes, such as synaptogenic modulator spare and transcriptional factors (emx2, lhx2, and hopx), was validated by qRT-PCR and immunostaining in brain sections. Interestingly, cortical or subcortical astrocytes selectively promote neurite growth and synaptic activity of neurons only from the same region in mismatched cocultures, exhibiting regionmatched astrocyte to neuron communication. Overall, these results generated new molecular signature of astrocyte types in the adult CNS, providing insights into their origin and functional diversity. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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