1. Direct and Single-Molecule Visualization of the Solution-State Structures of G-Hairpin and G-Triplex Intermediates.
- Author
-
Rajendran, Arivazhagan, Endo, Masayuki, Hidaka, Kumi, and Sugiyama, Hiroshi
- Subjects
- *
HAIRPIN (Genetics) , *MOLECULAR structure of nucleic acids , *INTERMEDIATES (Chemistry) , *ORGANIC synthesis , *ATOMIC force microscopy - Abstract
We present the direct and single-molecule visualization of the in-pathway intermediates of the G-quadruplex folding that have been inaccessible by any experimental method employed to date. Using DNA origami as a novel tool for the structural control and high-speed atomic force microscopy (HS-AFM) for direct visualization, we captured images of the unprecedented solution-state structures of a tetramolecular antiparallel and (3+1)-type G-quadruplex intermediates, such as G-hairpin and G-triplex, with nanometer precision. No such structural information was reported previously with any direct or indirect technique, solution or solid-state, single-molecule or bulk studies, and at any resolution. Based on our results, we proposed a folding mechanism of these G-quadruplexes. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF