1. DNA Interaction with Palladium Chelates of Biogenic Polyamines Using Atomic Force Microscopy and Voltammetric Characterization.
- Author
-
Corduneanu, O., Chiorcea-Paquim, A.-M., Diculescu, V., Fiuza, S. M., Marques, M. P. M., and Oliveira-Brett, A. M.
- Subjects
- *
SPERMINE , *PALLADIUM compounds , *ATOMIC force microscopy , *VOLTAMMETRY - Abstract
The interaction of double-stranded DNA with two poly-nuclear Pd(II) chelates with the biogenic polyamines spermidine (Spd) and spermine (Spm), Pd(II)-Spd and Pd(II)-Spm, as well as with the free ligands Spd and Spm, was studied using atomic force microscopy (AFM) at a highly oriented pyrolytic graphite (HOPG) surface, volta-mmetry at a glassy carbon (GC) electrode, and gel elec-trophoresis. The AFM and voltammetric results showed that the interaction of Spd and Spm with DNA occurred even for a low concentration of polyamines and caused no oxidative damage to DNA. The Pd(II)-Spd and Pd(II)-Spm complexes were found to induce greater morphological changes in the dsDNA conformation, when compared with their ligands. The interaction was specific, inducing distortion and local denaturation of the B-DNA structure with release of some guanine bases. The DNA strands partially opened give rise to palladium intra- and interstrand cross-links, leading to the formation of DNA adducts and aggregates, particularly in the case of the Pd(II) Spd complex. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF