1. Resveratrol inhibits plasma membrane Ca 2+ -ATPase inducing an increase in cytoplasmic calcium
- Author
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Richard Oblad, Jason Kenealey, Joshua Allen Peterson, and Jeffrey Chad Mecham
- Subjects
0301 basic medicine ,SERCA ,Fura-2 ,biology ,ATPase ,Biophysics ,Calcium signaling ,Resveratrol ,Biochemistry ,Calcium in biology ,Cell biology ,Plasma membrane Ca2+-ATPase ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,BAPTA ,biology.protein ,Plasma membrane Ca2+ ATPase - Abstract
Plasma membrane Ca2+-ATPase (PMCA) plays a vital role in maintaining cytosolic calcium concentration ([Ca2+]i). Given that many diseases have modified PMCA expression and activity, PMCA is an important potential target for therapeutic treatment. This study demonstrates that the non-toxic, naturally-occurring polyphenol resveratrol (RES) induces increases in [Ca2+]i via PMCA inhibition in primary dermal fibroblasts and MDA-MB-231 breast cancer cells. Our results also illustrate that RES and the fluorescent intracellular calcium indicator Fura-2, are compatible for simultaneous use, in contrast to previous studies, which indicated that RES modulates the Fura-2 fluorescence independent of calcium concentration. Because RES has been identified as a PMCA inhibitor, further studies may be conducted to develop more specific PMCA inhibitors from RES derivatives for potential therapeutic use.
- Published
- 2016
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