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The attitudes of pharmacists in three practice settings toward a set of attributes associated with professionalism were studied. A questionnaire that included the 40-item Shack and Hepler professionalism instrument was mailed to 1999 pharmacists in Ohio. Responses from 617 pharmacists who worked in hospital, independent community, or chain community pharmacy practice were used. There were significant differences between the responses of pharmacists who worked in the different practice settings (multivariate analysis of variance, p less than 0.01). However, the practice setting accounted for less than 10% of the variance in the responses. Statistically significant differences were also observed when the pharmacists were grouped according to sex, years since graduation, degree, and job position; however, less than 8% of the variance in the responses could be explained by the demographic variables. There did not appear to be any practical differences in beliefs about professional attributes among pharmacists working in hospital, independent community, and chain community practice settings.