1. Serum and cerebrospinal fluid antibodies to Nogo-A in patients with multiple sclerosis and acute neurological disorders.
- Author
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Reindl M, Khantane S, Ehling R, Schanda K, Lutterotti A, Brinkhoff C, Oertle T, Schwab ME, Deisenhammer F, Berger T, and Bandtlow CE
- Subjects
- Acute Disease, Adult, Aged, Animals, Autoantibodies biosynthesis, Autoantibodies metabolism, Brain immunology, Brain metabolism, CHO Cells, Cells, Cultured, Central Nervous System Diseases blood, Central Nervous System Diseases cerebrospinal fluid, Central Nervous System Diseases immunology, Cricetinae, Humans, Immunoglobulin G biosynthesis, Immunoglobulin G cerebrospinal fluid, Immunoglobulin M biosynthesis, Immunoglobulin M blood, Middle Aged, Multiple Sclerosis blood, Multiple Sclerosis cerebrospinal fluid, Nogo Proteins, Oligodendroglia immunology, Oligodendroglia metabolism, Rats, Transfection, Autoantibodies blood, Autoantibodies cerebrospinal fluid, Autoantigens immunology, Multiple Sclerosis immunology, Myelin Proteins immunology
- Abstract
Nogo-A is a protein associated with central nervous system (CNS) myelin thought to impair regenerative responses and to suppress sprouting and plastic changes of synaptic terminals. In this study, we report that serum IgM autoantibodies to the recombinant large N-terminal inhibitory domain of Nogo-A are a frequent finding in multiple sclerosis (MS) and acute inflammatory (IND) and non-inflammatory neurological diseases (OND), but not in neurodegenerative diseases (ND), systemic inflammatory disease and healthy controls. Furthermore, we demonstrate intrathecal production of anti-Nogo-A antibodies measured by increased IgG indices. Intrathecal anti-Nogo antibodies were significantly more frequent in patients with relapsing-remitting as compared to chronic progressive (CP) MS. We also found a highly significant negative correlation of these antibody responses with age indicating that they are more frequent in younger patients. We finally demonstrate that human anti-Nogo-A antibodies recognize native Nogo-A in brain extracts, oligodendrocytes and cells expressing human Nogo-A.
- Published
- 2003
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