1. Interaction of thyroid-stimulating antibody with Graves' thyroid-stimulating hormone-binding antibody.
- Author
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Ochi Y, Kajita Y, Hamazu M, and Nagata A
- Subjects
- Antibodies, Anti-Idiotypic blood, Antibodies, Anti-Idiotypic immunology, Antibody Affinity, Autoantibodies blood, Graves Disease blood, Humans, Immunoglobulins, Thyroid-Stimulating blood, Immunoglobulins, Thyroid-Stimulating isolation & purification, Receptors, Thyrotropin blood, Thyrotropin blood, Autoantibodies immunology, Graves Disease immunology, Immunoglobulins, Thyroid-Stimulating immunology, Receptors, Thyrotropin immunology, Thyrotropin immunology
- Abstract
Objective: Evidence of anti-thyroid-stimulating hormone (TSH) antibody in Graves' serum has been reported. We found that extremely high Graves' anti-TSH antibodies neutralized other Graves' thyroid-stimulating antibody (TSAb) activity., Method: TSAb-IgG was affinity-purified by Sepharose-bound Graves' anti-TSH antibody (extremely high)., Result: The thyroid-stimulating activity in affinity-purified TSAb-IgG increased about 4-5 times compared to that before purification. TSH-binding inhibitory immunoglobulin (TBII) activity in affinity-purified TSAb-IgG also increased using TSH receptor-coated tube assay. A similar increase of thyroid-stimulating activity accompanied with TBII activity was also observed in affinity-purified TSAb-IgG-F(ab')(2)., Conclusion: This suggests the possibility that either TSAb may be an anti-idiotypic antibody against anti-TSH antibody or anti-TSH antibody may be an anti-idiotypic antibody against anti-TSH receptor antibody., (Copyright 2003 S. Karger AG, Basel)
- Published
- 2003
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