1. Clinical pilot study on microfluidic automation of IGH-VJ library preparation for next generation sequencing.
- Author
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Hess, Jacob F., Kotrová, Michaela, Fricke, Birgit, Songia, Simona, Rigamonti, Silvia, Cavagna, Roberta, Tosi, Manuela, Paust, Nils, Langerak, Anton W., Spinelli, Orietta, Cazzaniga, Giovanni, Brüggemann, Monika, and Hutzenlaub, Tobias
- Subjects
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NUCLEOTIDE sequencing , *LIBRARY automation , *AUTOMATION software , *AUTOMATION , *T-cell receptor genes , *PILOT projects , *WHOLE genome sequencing - Abstract
This document is a letter published in the journal Clinical Chemistry & Laboratory Medicine. It discusses a clinical pilot study on the automation of library preparation for next-generation sequencing (NGS) using microfluidics. The study focuses on the screening of somatic rearrangements of Immunoglobulin (IG) genes in patients with acute lymphoblastic leukemia (ALL). The researchers used a centrifugal microfluidic cartridge and a modified commercially available player for the automation process. The results showed that the automated library preparation using microfluidics was comparable to manual library preparation in terms of coverage and detection of IGH-VJ rearrangements. The study suggests that centrifugal microfluidic automation can reduce complexity and staff hands-on time in a clinical setup for library preparation. However, the choice of automation system depends on the throughput numbers of the laboratory. The researchers also mention the possibility of manufacturing cartridges for other lymphoid malignancies and the potential for MRD quantification by NGS. [Extracted from the article]
- Published
- 2024
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