1. LRRC25 inhibits type I IFN signaling by targeting ISG15-associated RIG-I for autophagic degradation.
- Author
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Du Y, Duan T, Feng Y, Liu Q, Lin M, Cui J, and Wang RF
- Subjects
- Animals, COS Cells, Cell Line, Chlorocebus aethiops, Cytokines metabolism, HEK293 Cells, Humans, Membrane Proteins genetics, Protein Binding immunology, RNA Interference, RNA, Small Interfering genetics, Receptors, Immunologic, Signal Transduction immunology, Ubiquitins metabolism, Vesicular stomatitis Indiana virus immunology, Autophagy immunology, DEAD Box Protein 58 metabolism, Interferon Type I immunology, Membrane Proteins metabolism, RNA-Binding Proteins metabolism
- Abstract
The RIG-I-like receptors (RLRs) are critical for protection against RNA virus infection, and their activities must be stringently controlled to maintain immune homeostasis. Here, we report that leucine-rich repeat containing protein 25 (LRRC25) is a key negative regulator of RLR-mediated type I interferon (IFN) signaling. Upon RNA virus infection, LRRC25 specifically binds to ISG15-associated RIG-I to promote interaction between RIG-I and the autophagic cargo receptor p62 and to mediate RIG-I degradation via selective autophagy. Depletion of either LRRC25 or ISG15 abrogates RIG-I-p62 interaction as well as the autophagic degradation of RIG-I. Collectively, our findings identify a previously unrecognized role of LRRC25 in type I IFN signaling activation by which LRRC25 acts as a secondary receptor to assist RIG-I delivery to autophagosomes for degradation in a p62-dependent manner., (© 2017 The Authors.)
- Published
- 2018
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