19 results on '"Fracasso, Tony"'
Search Results
2. Fatal Ogilvie’s syndrome after hip surgery and review of the literature
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Aguiar, Diego, Fracasso, Tony, and Lardi, Christelle
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- 2022
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3. Fatal diving: could it be an immersion pulmonary edema? Case report
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Evain, France, Louge, Pierre, Pignel, Rodrigue, Fracasso, Tony, and Rouyer, Frédéric
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- 2022
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4. Hidden lesions: a case of burnt remains.
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Moghaddam, Negahnaz, Campana, Lorenzo, Abegg, Claudine, Vilarino, Raquel, Voland, Christelle, Dedouit, Fabrice, Genet, Pia, and Fracasso, Tony
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AUTOPSY ,FORENSIC pathologists ,BLUNT trauma ,ANTHROPOLOGY ,HUMAN skeleton ,ANTHROPOLOGISTS ,ARCHAEOLOGICAL human remains - Abstract
One of the many challenging cases that forensic pathologists, anthropologists, and forensic imaging experts have to face are burnt human remains. Perpetrators frequently attempt to hide/destroy evidence and make the body unidentifiable by exposing it to fire. We present a case of a partially burnt body found in an apartment after an explosion. First, multidetector computed tomography (MDCT) images and the following autopsy revealed several lesions on the cranium. Forensic anthropologists were involved in order to specify the aetiology of the lesions observed on the cranium. Through an interdisciplinary approach bringing together MDCT scans, 3D surface scans, and anthropological analysis, it was possible to answer the questions raised during the autopsy. Analyses demonstrated that there were signs of blunt force trauma on the cranium vault that the perpetrator likely attempted to hide by exposing the body to fire. This case demonstrates the importance of close collaboration between forensic anthropologists, imaging experts, and forensic pathologists. This multidisciplinary approach allows for a better, more complete reconstitution of forensic cases. Key points The analyses of burnt human remains are one of the many challenging tasks that forensic pathologists and anthropologists have to face. We present an occurrence of a partially burnt body after an explosion and forensic anthropologists were asked whether the nature of the lesions observed on the cranium could be further specified. Anthropological analyses of the skull were consistent with the radiological and autopsy report. It was possible to reconstruct the various lesions on the dry bone. The case demonstrates the importance of an interdisciplinary approach and the close collaboration between forensic anthropologists, imaging experts, and forensic pathologists. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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5. A forensic case of hydranencephaly in a preterm neonate fully documented by postmortem imaging techniques.
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Egger, Coraline, Dédouit, Fabrice, Schrag, Bettina, Hanquinet, Sylviane, and Fracasso, Tony
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MAGNETIC resonance imaging ,AUTOPSY ,CEREBRAL hemispheres ,CENTRAL nervous system ,NEWBORN infants ,FORENSIC pathology ,CAUSES of death - Abstract
The authors present a medico-legal autopsy case of hydranencephaly in a male preterm newborn, fully documented by postmortem unenhanced and enhanced imaging techniques (postmortem computed tomography and postmortem magnetic resonance imaging). Hydranencephaly is a congenital anomaly of the central nervous system, consisting in almost complete absence of the cerebral hemispheres and replacement of the cerebral parenchyma by cerebrospinal fluid, rarely encountered in forensic medical practice. A premature baby was born during the supposed 22nd and 24th week of pregnancy in the context of a denial of pregnancy without any follow-up. The newborn died a few hours after birth and medico-legal investigations were requested to determine the cause of death and exclude the intervention of a third person in the lethal process. The external examination revealed neither traumatic nor malformative lesions. Postmortem imaging investigations were typical of hydranencephaly, and conventional medico-legal autopsy, neuropathological examination, and histological examination confirmed a massive necrotic-haemorrhagic hydranencephaly. This case represents in itself an association of out-of-the-ordinary elements making it worthy of interest. Key Points Postmortem unenhanced and enhanced imaging techniques (computed tomography and magnetic resonance imaging) were performed as complementary examination to conventional medico-legal investigations. Postmortem angiography of a preterm newborn is possible with catheterization of the umbilical blood vessels. Hydranencephaly is a congenital anomaly of the central nervous system, consisting in almost complete absence of the cerebral hemispheres and replacement of the brain by cerebrospinal fluid, for which several aetiologies have been postulated. [ABSTRACT FROM AUTHOR]
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- 2023
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6. Traumatic injuries after mechanical cardiopulmonary resuscitation (LUCAS™2): a forensic autopsy study
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Lardi, Christelle, Egger, Coraline, Larribau, Robert, Niquille, Marc, Mangin, Patrice, and Fracasso, Tony
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- 2015
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7. Postmortem coronary artery calcium score in cases of myocardial infarction.
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Michaud, Katarzyna, Magnin, Virginie, Faouzi, Mohamed, Fracasso, Tony, Aguiar, Diego, Dedouit, Fabrice, and Grabherr, Silke
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MYOCARDIAL infarction ,CORONARY arteries ,CORONARY artery disease ,COMPUTED tomography ,CAUSES of death ,AUTOPSY - Abstract
Sudden cardiac death (SCD) related to atherosclerotic coronary artery disease (ACAD) resulting in myocardial infarction is the most prevalent cause of death in western countries. In clinical practice, coronary artery calcium score (CACS) is considered an independent predictor of coronary events, closely related to atherosclerotic burden and is quantified radiologically by the Agatston score being calculated through computed tomography. Postmortem computed tomography (PMCT) allows the visualization and quantification of coronary calcifications before the autopsy. However, it was reported that some patients who died from severe ACAD had a zero CACS in PMCT. In this study, a retrospective evaluation of CACS in adult's myocardial infarction cases related to ACAD, with available CACS and histological slides of coronary arteries, was performed in order to gain a deeper understanding of coronary calcifications and their role in myocardial infarction cases. The CACS was calculated by using the software Smartscore 4.0 after the radiological examination on a 64-row CT unit using a specific cardiac protocol. Thirty-six cases were identified out of 582 autopsies, recorded during a 2-year study period (29 men, 7 women; age 56.3 ± 11.7). CACS was 0–10 in 5 cases (5 men, 44.8 ± 13.7), 11–100 in 8 cases (6 men, 2 women, 53.1 ± 7.7), 101–400 in 13 cases (11 men, 2 women, 57.4 ± 9.6), and > 400 in 10 cases (9 men, 1 woman, 63.1 ± 11.9). Coronary thrombosis was found in 28 cases, histologically identified as plaque erosions in 6 cases and as plaque ruptures in 22 cases. Statistical analyses showed that CACS increases significantly with age (p-value < 0.05) and does not show significant correlation with gender, body weight, body mass index, and heart weight. CACS was significantly higher in plaque ruptures than in plaque erosions (p-value < 0.01). Zero or low CACS on unenhanced PMCT cannot exclude the presence of myocardial infarction related to ACAD. This paradoxical discrepancy between imaging and autopsy findings can be explained considering the histological aspect of fatal coronary plaques. [ABSTRACT FROM AUTHOR]
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- 2021
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8. Inside the lungs of COVID-19 disease.
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Aguiar, Diego, Lobrinus, Johannes Alexander, Schibler, Manuel, Fracasso, Tony, and Lardi, Christelle
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LUNG diseases ,COVID-19 ,FORENSIC pathology ,PULMONARY fibrosis ,SARS-CoV-2 ,AUTOPSY - Abstract
In the setting of the coronavirus disease 2019 (COVID-19) pandemic, only few data regarding lung pathology induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is available, especially without medical intervention interfering with the natural evolution of the disease. We present here the first case of forensic autopsy of a COVID-19 fatality occurring in a young woman, in the community. Diagnosis was made at necropsy and lung histology showed diffuse alveolar damage, edema, and interstitial pneumonia with a geographically heterogeneous pattern, mostly affecting the central part of the lungs. This death related to COVID-19 pathology highlights the heterogeneity and severity of central lung lesions after natural evolution of the disease. [ABSTRACT FROM AUTHOR]
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- 2020
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9. Intimomedial mucoid arterial degeneration, a rare arterial disorder of forensic significance.
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Wiskott, Kim, Genet, Pia, Lobrinus, Johannes A., Fracasso, Tony, and Lardi, Christelle
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AUTOPSY ,HYPERTENSION ,AORTIC aneurysms ,ILIAC artery ,AORTA ,DEGENERATION (Pathology) ,RENAL artery - Abstract
The body of a 43-year-old African woman with a history of aortic aneurysm and hypertension was forensically investigated after her sudden death. The cause of death was related to a cardiac tamponade due to a ruptured aneurysm of the ascending aorta. Post-mortem gross examination showed an abnormal whitish discoloration of the intima with fibrous thickening of the aortic wall. Several arteries (left main and circumflex coronaries, carotid, renal and iliac arteries) showed similar features. Upon histological examination, the aortic aneurysm as well as the other arteries sampled showed mucoid degeneration, excess mucopolysaccharides and pools of mucin inside the intima and the media associated with collagen and elastic fiber destruction and loss of smooth muscle cells. This pattern strongly suggested the diagnosis of intimomedial mucoid degeneration (IMMD), a rare arterial disorder consisting of a progressive deposition of mucin into the intima and media, with a strong prevalence in middle-aged black African females with high blood pressure. In addition to the typical features of IMMD, histological examination of the ascending aorta showed a thickening of the adventita with sparse mixed inflammatory infiltrates and fibrosis, suggesting an additional chronic infectious aortitis. No infectious agent was detected. The body of literature on IMMD is reviewed and the origin of death is discussed in this case report. [ABSTRACT FROM AUTHOR]
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- 2019
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10. The Reliability of Facial Recognition of Deceased Persons on Photographs.
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Caplova, Zuzana, Obertova, Zuzana, Gibelli, Daniele M., Mazzarelli, Debora, Fracasso, Tony, Vanezis, Peter, Sforza, Chiarella, and Cattaneo, Cristina
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FORENSIC sciences ,FORENSIC anthropology ,FACE perception ,IMMIGRANTS ,AUTOPSY - Abstract
In humanitarian emergencies, such as the current deceased migrants in the Mediterranean, antemortem documentation needed for identification may be limited. The use of visual identification has been previously reported in cases of mass disasters such as Thai tsunami. This pilot study explores the ability of observers to match unfamiliar faces of living and dead persons and whether facial morphology can be used for identification. A questionnaire was given to 41 students and five professionals in the field of forensic identification with the task to choose whether a facial photograph corresponds to one of the five photographs in a lineup and to identify the most useful features used for recognition. Although the overall recognition score did not significantly differ between professionals and students, the median scores of 78.1% and 80.0%, respectively, were too low to consider this method as a reliable identification method and thus needs to be supported by other means. [ABSTRACT FROM AUTHOR]
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- 2017
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11. Apparent versus true gene expression changes of three hypoxia-related genes in autopsy derived tissue and the importance of normalisation.
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Huth, Antje, Vennemann, Benedikt, Fracasso, Tony, Lutz-Bonengel, Sabine, and Vennemann, Marielle
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GENE expression ,HYPOXEMIA ,AUTOPSY ,MESSENGER RNA ,POLYMERASE chain reaction - Abstract
The aim of our work was to show how a chosen normal-isation strategy can affect the outcome of quantitative gene expression studies. As an example, we analysed the expression of three genes known to be upregulated under hypoxic conditions: HIF1A, VEGF and SLC2A1 ( GLUT1). Raw RT-qPCR data were normalised using two different strategies: a straightforward normalisation against a single reference gene, GAPDH, using the 2 algorithm and a more complex normalisation against a normalisation factor calculated from the quantitative raw data from four previously validated reference genes. We found that the two different normalisation strategies revealed contradicting results: normalising against a validated set of reference genes revealed an upregulation of the three genes of interest in three post-mortem tissue samples (cardiac muscle, skeletal muscle and brain) under hypoxic conditions. Interestingly, we found a statistically significant difference in the relative transcript abundance of VEGF in cardiac muscle between donors who died of asphyxia versus donors who died from cardiac death. Normalisation against GAPDH alone revealed no upregulation but, in some instances, a downregulation of the genes of interest. To further analyse this discrepancy, the stability of all reference genes used were reassessed and the very low expression stability of GAPDH was found to originate from the co-regulation of this gene under hypoxic conditions. We concluded that GAPDH is not a suitable reference gene for the quantitative analysis of gene expression in hypoxia and that validation of reference genes is a crucial step for generating biologically meaningful data. [ABSTRACT FROM AUTHOR]
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- 2013
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12. RNA integrity in post-mortem samples: influencing parameters and implications on RT-qPCR assays.
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Koppelkamm, Antje, Vennemann, Benedikt, Lutz-Bonengel, Sabine, Fracasso, Tony, and Vennemann, Marielle
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MESSENGER RNA ,AUTOPSY ,GENE expression ,BODY mass index ,POLYMERASE chain reaction - Abstract
Messenger RNA (mRNA) profiling in post-mortem human tissue might reveal information about gene expression at the time point of death or close to it. When working with post-mortem human tissue, one is confronted with a natural RNA degradation caused by several parameters which are not yet fully understood. The aims of the present study were to analyse the influence of impaired RNA integrity on the reliability of quantitative gene expression data and to identify ante- and post-mortem parameters that might lead to reduced RNA integrities in post-mortem human brain, cardiac muscle and skeletal muscle tissues. Furthermore, this study determined the impact of several parameters like type of tissue, age at death, gender and body mass index (BMI), as well as duration of agony, cause of death and post-mortem interval on the RNA integrity. The influence of RNA integrity on the reliability of quantitative gene expression data was analysed by generating degradation profiles for three gene transcripts. Based on the deduced cycle of quantification data, this study shows that reverse transcription quantitative polymerase chain reaction (RT-qPCR) performance is affected by impaired RNA integrity. Depending on the transcript and tissue type, a shift in cycle threshold values of up to two cycles was observed. Determining RNA integrity number of 136 post-mortem samples revealed significantly different RNA qualities among the three tissue types with brain revealing significantly lower integrities compared to skeletal and cardiac muscle. The body mass index was found to influence RNA integrity in skeletal muscle tissue (M. iliopsoas). Samples originating from deceased with a BMI > 25 were of significantly lower integrity compared to samples from normal weight donors. Correct data normalisation was found to partly diminish the effects caused by impaired RNA quality. Nevertheless, it can be concluded that in post-mortem tissue with low RNA integrity numbers, the detection of large differences in gene expression activities might still be possible, whereas small expression differences are prone to misinterpretation due to degradation. Thus, when working with post-mortem samples, we recommend generating degradation profiles for all transcripts of interest in order to reveal detection limits of RT-qPCR assays. [ABSTRACT FROM AUTHOR]
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- 2011
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13. Petechial bleedings in sudden infant death.
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Fracasso, Tony, Vennemann, Mechtild, Klöcker, Mirjam, Bajanowski, Thomas, Brinkmann, Bernd, and Pfeiffer, Heidi
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SUDDEN infant death syndrome , *HEMORRHAGE , *DISEASE prevalence , *ASPHYXIA , *CAUSES of death , *AUTOPSY - Abstract
The autopsy reports of 484 cases of deceased infants (201 females, 283 males) were analysed retrospectively for the existence of external and internal petechial bleedings (PET). The cases were divided into five groups on the basis of the cause of death (sudden infant death syndrome, sepsis, airway infections, asphyxia and trauma). Internal PET (pleural, pericardial, epicardial, thymic and peritoneal) were observed in each group with a lower prevalence in cases of trauma. The highest prevalence of external (cutaneous and conjunctival) PET was detected in cases of asphyxia (38% and 31%, respectively). However, even if with low prevalence, such bleedings were detected in every group. Factors like sex, age, cardiopulmonary resuscitation and its duration did not influence the presence of PET. The detection of external PET at autopsy is a suspicious finding that suggests asphyxia. Because of the possible natural origin of these bleedings, the medicolegal investigation has to be as complete as possible and has to include histology as mandatory. [ABSTRACT FROM AUTHOR]
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- 2011
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14. Delayed asphyxia due to inhalation injury.
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Fracasso, Tony and Schmeling, Andreas
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TOXICOLOGY of poisonous gases , *HISTOLOGY , *ASPHYXIA , *CAUSES of death , *AUTOPSY , *FORENSIC pathology - Abstract
We report the case of a 53-year-old man who was found dead in his apartment 2 days after a little fire during which he had suffered from second to third degree burns at 2% of the body surface, also involving the skin around the mouth and the nose. At autopsy, severe acute emphysema and mucopurulent obstructive laryngotracheobronchitis were observed. The histology showed desquamative loss of the respiratory epithelium up to the middle bronchi; the bronchial lumen was filled with clumps of mucopurulent secretions mixed with necrotic epithelial cells. The cause of death was a delayed asphyxia due to inhalation injury. [ABSTRACT FROM AUTHOR]
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- 2011
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15. Ultrasound-accelerated formalin fixation improves the preservation of nucleic acids extraction in histological sections.
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Fracasso, Tony, Heinrich, Marielle, Hohoff, Carsten, Brinkmann, Bernd, and Pfeiffer, Heidi
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FORMALDEHYDE , *NUCLEIC acids , *AUTOPSY , *DNA , *RNA - Abstract
The aim of the present study was to examine an ultrasound-accelerated fixation technique that reduces the exposure time of the tissue to formaldehyde with respect to the analysis of nucleic acids. We extracted and analysed DNA and RNA from three series of autopsy specimens from five routine cases. Two series were shortly fixed in 4% buffered formalin (15 and 30 min, respectively) whilst being irradiated with high-frequency, high-intensity ultrasound. The last series (control) was routinely fixed in 4% buffered formalin for 24–48 h without irradiation. Although sufficient amounts of DNA of good quality could be extracted and amplified from all three series, the peak heights obtained from conventional fixation were smaller and allele dropout occurred more often, especially for the longer amplicons. RNA yield depended on the fixation procedure, i.e. the shortest fixation time led to the highest RNA yield and quality. No differences were observed with regard to the quality of the histological slides both with conventional and immunohistochemical staining methods. Keeping in mind the increasing need for molecular diagnosis, this fixation technique can be useful to ensure stable quality of nucleic acids in archived autopsy specimens. [ABSTRACT FROM AUTHOR]
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- 2009
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16. Unassisted smothering in a pillow.
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Schmeling, Andreas, Fracasso, Tony, Pragst, Fritz, Tsokos, Michael, and Wirth, Ingo
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PARANOID schizophrenia , *AUTOPSY , *ASPHYXIA , *ANTIPSYCHOTIC agents , *BRUISES - Abstract
We report the case of a 33-year-old man admitted to a psychiatric hospital because of paranoid schizophrenia. The man was found dead lying in his bed with the face pressed against a pillow and with elevated buttocks. The autopsy did not reveal a cause of death. The histological findings displayed the signs of the haemorrhagic–dysoric syndrome with acute emphysema; these findings are pathognomonic of obstructive asphyxia. The adverse effects of the neuroleptics demonstrated by the toxicological findings may have accelerated the loss of consciousness and facilitated the unusual position of the body. On the basis of the clinical history, the autopsy findings, the histological features and the toxicological results, asphyxia due to smothering was diagnosed as the cause of death. [ABSTRACT FROM AUTHOR]
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- 2009
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17. High-sensitive cardiac troponin hs-TnT levels in sudden deaths related to atherosclerotic coronary artery disease.
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Beausire, Tim, Faouzi, Mohamed, Palmiere, Cristian, Fracasso, Tony, and Michaud, Katarzyna
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TROPONIN , *SUDDEN death , *CORONARY disease , *ATHEROSCLEROSIS , *AUTOPSY , *REGRESSION analysis , *LOGISTIC regression analysis , *RETROSPECTIVE studies , *CASE-control method - Abstract
Introduction: Ischemic heart disease (IHD) related to atherosclerotic coronary artery disease (CAD) is one of the most prevalent causes of death in Europe. Postmortem evaluation of IHD remains a challenge because of possible non-specific autopsy finding in some autopsy cases, especially in early myocardial ischemia. High-sensitive cardiac troponin T (hs-TnT) is used today in clinical practice as the "gold standard" to diagnose the myocardial ischemia, and might also be applied as an ancillary tool for post-mortem evaluation.Purpose: The goal of this study is to evaluate the diagnostic value of post-mortem serum hs-TnT assay in cases of sudden death related to IHD. We will also investigate the influence of cardiopulmonary resuscitation (CPR) attempts on post-mortem hs-TnT levels.Methods: The hs-TnT values in serum were retrospectively analysed in 85 autopsy data. 52 cases with clinical history and morphological results suggesting cardiac ischemia were included in the study group (mean age 53.5; age range 34-75) and 33 cases in the control group (mean age 40.4; age range 15-69). The group's statistical comparison was performed using logistic regression model.Results: Our study showed a significant non-linear association between hs-TnT serum values and post-mortem diagnosis of sudden deaths related to IHD (p-value 0.005). The shape of the relationship is showing that the probability of death due to IHD increases quickly with a light level of hs-TnT (maximum around 90ng/L) then decreases slightly while remaining at high in values. No significant difference in the hs-TnT serum values was found between the CPR and the non-CPR cases (p-value 0.304).Conclusion: The measurement of hs-TnT serum values might be considered as an ancillary tool for the evaluation of death related to IHD, while taking necessary precautions in the interpretation of the results. [ABSTRACT FROM AUTHOR]- Published
- 2018
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18. Summary statistics for drugs and alcohol concentration recovered in post-mortem femoral blood in Western Switzerland.
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Lefrancois, Elodie, Reymond, Naomi, Thomas, Aurélien, Lardi, Christelle, Fracasso, Tony, and Augsburger, Marc
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BLOOD alcohol , *AUTOPSY , *DEATH , *ALCOHOLIC intoxication , *POSTMORTEM changes , *ARTHRITIS Impact Measurement Scales , *DRUGS , *ETHANOL , *CENTRAL nervous system depressants , *FORENSIC toxicology - Abstract
In post-mortem investigations of fatal intoxication, it is challenging to determine which drug(s) were responsible for the death, and which drugs did not. This study aims to provide post-mortem femoral blood drug levels in lethal intoxication and in post-mortem control cases, where the cause of death was other than intoxication. The reference values could assist in the interpretation of toxicological results in the routine casework. To this end, all post-mortem toxicological results in femoral blood from 2011 to 2017 in Western Switzerland were considered. A full autopsy with systematic toxicological analysis (STA) was conducted in all cases. Results take into account the cause of death classified into one of four categories (as published by Druid and colleagues): I) certified intoxication by one substance alone, IIa) certified intoxication by more than one substance, IIb) certified other causes of death with incapacitation due to drugs, and III) certified other causes of death without incapacitation due to drugs. This study includes 1 990 post-mortem cases where femoral blood was analysed. The material comprised 619 women (31%) and 1 371 men (69%) with a median age of 50 years. The concentrations of the 32 most frequently recorded substances as well as alcohol are discussed. These include 6 opioids and opiates, 3 antidepressants, 6 neuroleptics and hypnotics, 1 barbiturate, 11 benzodiazepines (and related drugs), 2 amphetamine-type stimulants, cocaine, paracetamol, and tetrahydrocannabinol (THC). The most common substances that caused intoxication alone were morphine, methadone, ethanol, tramadol, and cocaine. The post-mortem concentration ranges for all substance are categorized as I, IIa, IIb, or III. Statistical post-mortem reference concentrations for drugs are discussed and compared with previously published concentrations. This study shows that recording and classifying cases is time-consuming, but it is rewarding in a long-term perspective to achieve a more reliable information about fatal and non-fatal blood concentrations. [ABSTRACT FROM AUTHOR]
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- 2021
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19. The modern holy shroud
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Schrag, Bettina, Pitteloud, Sophie, Horisberger, Beat, Fracasso, Tony, and Mangin, Patrice
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HOLY Shroud , *EXPRESS highways , *PEDESTRIANS , *FORENSIC pathologists , *WITNESSES , *AUTOPSY - Abstract
Abstract: Testimonies disclosed that a 44-year-old pedestrian was struck head-on by a truck while she was roaming on the motorway; at the time of collision, the truck was travelling at a speed of about 90km/h. In the second phase of the collision, the pedestrian was projected about 100m before her body was run over by the truck and then by a car. The autopsy revealed extensive mutilations, making it impossible to verify the testimonies of witnesses to the collision as regards the pedestrian''s position at the moment of the first impact. However, the reports produced by the technical expert and the forensic pathologist were able to confirm the testimonies, based on an impact zone on the front panel of the cab of the truck, where part of the pedestrian''s face was reproduced like a “modern holy shroud”. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
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