1. Down-regulation of a single auxin efflux transport protein in tomato induces precocious fruit development
- Author
-
Virginie Garcia, Catherine Deborde, Mariusz Kowalczyk, Koh Aoki, Antonio Granell, Martine Lemaire-Chamley, Catherine Bellini, Alisdair R. Fernie, Johann Petit, Fabien Mounet, Mickaël Maucourt, Kentaro Yano, Johannes Rohrmann, Hélène Bergès, Christophe Rothan, Annick Moing, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Department of Plant Physiology, Umea Plant Science Centre, Umeå University-Umeå University, Centre National de Ressources Génomiques Végétales (CNRGV), Institut National de la Recherche Agronomique (INRA), Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Region Aquitaine [20051303006ABC], EUSOL [FOOD-CT-2006-016214], Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, Umea Plant Science Center (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences (SLU)-Swedish University of Agricultural Sciences (SLU), COGNITIQUE, Laboratoire de l'intégration, du matériau au système (IMS), Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Université Sciences et Technologies - Bordeaux 1, Meiji University, Kazusa DNA Research Institute (KDRI), Universitat Politècnica de València (UPV), and Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)
- Subjects
0106 biological sciences ,Auxin efflux ,Physiology ,[SDV]Life Sciences [q-bio] ,Plant Science ,Parthenocarpy ,Plant Roots ,01 natural sciences ,CRABS-CLAW ,Solanum lycopersicum ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Biologiska vetenskaper ,PIN proteins ,Phylogeny ,ComputingMilieux_MISCELLANEOUS ,transcription factor ,Oligonucleotide Array Sequence Analysis ,Plant Proteins ,Regulator gene ,chemistry.chemical_classification ,0303 health sciences ,Bud ,Auxin efflux transport protein (PIN) ,Gene Expression Regulation, Developmental ,food and beverages ,Biological Sciences ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,Plants, Genetically Modified ,Up-Regulation ,Cell biology ,Phenotype ,RNA Interference ,fruit set ,tomato (Solanum lycopersicum) ,Research Paper ,Signal Transduction ,Down-Regulation ,Flowers ,Biology ,03 medical and health sciences ,Auxin ,Botany ,MADS-BOX ,030304 developmental biology ,Indoleacetic Acids ,Auxin homeostasis ,Gene Expression Profiling ,fungi ,Biological Transport ,biology.organism_classification ,chemistry ,Fruit ,parthenocarpy ,Solanum ,Transcription Factors ,010606 plant biology & botany - Abstract
Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; The PIN-FORMED (PIN) auxin efflux transport protein family has been well characterized in the model plant Arabidopsis thaliana, where these proteins are crucial for auxin regulation of various aspects of plant development. Recent evidence indicates that PIN proteins may play a role in fruit set and early fruit development in tomato (Solanum lycopersicum), but functional analyses of PIN-silenced plants failed to corroborate this hypothesis. Here it is demonstrated that silencing specifically the tomato SlPIN4 gene, which is predominantly expressed in tomato flower bud and young developing fruit, leads to parthenocarpic fruits due to precocious fruit development before fertilization. This phenotype was associated with only slight modifications of auxin homeostasis at early stages of flower bud development and with minor alterations of ARF and Aux/IAA gene expression. However, microarray transcriptome analysis and large-scale quantitative RT-PCR profiling of transcription factors in developing flower bud and fruit highlighted differentially expressed regulatory genes, which are potential targets for auxin control of fruit set and development in tomato. In conclusion, this work provides clear evidence that the tomato PIN protein SlPIN4 plays a major role in auxin regulation of tomato fruit set, possibly by preventing precocious fruit development in the absence of pollination, and further gives new insights into the target genes involved in fruit set.
- Published
- 2012
- Full Text
- View/download PDF