1. Risk Assessment for Highly Pathogenic Avian Influenza A(H5N6/H5N8) Clade 2.3.4.4 Viruses.
- Author
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Bui, Christine H. T., Kuok, Denise I. T., Hin Wo Yeung, Ka-Chun Ng, Chu, Daniel K. W., Webby, Richard J., Nicholls, John M., Peiris, J. S. Malik, Hui, Kenrie P. Y., Chan, Michael C. W., Yeung, Hin Wo, and Ng, Ka-Chun
- Subjects
H5N1 Influenza ,AVIAN influenza ,RISK assessment ,VIRUSES ,VIRAL tropism ,EPITHELIAL cells ,AVIAN influenza epidemiology ,BIRDS ,RESEARCH ,RESEARCH methodology ,MEDICAL cooperation ,EVALUATION research ,COMPARATIVE studies ,INFLUENZA ,INFLUENZA A virus, H1N1 subtype ,INFLUENZA A virus, H5N1 subtype - Abstract
The numerous global outbreaks and continuous reassortments of highly pathogenic avian influenza (HPAI) A(H5N6/H5N8) clade 2.3.4.4 viruses in birds pose a major risk to the public health. We investigated the tropism and innate host responses of 5 recent HPAI A(H5N6/H5N8) avian isolates of clades 2.3.4.4b, e, and h in human airway organoids and primary human alveolar epithelial cells. The HPAI A(H5N6/H5N8) avian isolates replicated productively but with lower competence than the influenza A(H1N1)pdm09, HPAI A(H5N1), and HPAI A(H5N6) isolates from humans in both or either models. They showed differential cellular tropism in human airway organoids; some infected all 4 major epithelial cell types: ciliated cells, club cells, goblet cells, and basal cells. Our results suggest zoonotic potential but low transmissibility of the HPAI A(H5N6/H5N8) avian isolates among humans. These viruses induced low levels of proinflammatory cytokines/chemokines, which are unlikely to contribute to the pathogenesis of severe disease. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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