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1. [Polyphotobiotin--a new reagent for introducing biotin into nucleic acids].

Author

Turchinskiĭ MF, Shcherbo SN, Knorre VD, Kolesnik TB, and Aleĭnik IuV

Subjects
Affinity Labels, Bacteriophages metabolism, Biotin chemistry, DNA Probes chemistry, DNA, Single-Stranded chemistry, DNA, Viral chemistry, Nucleic Acid Hybridization, Polymers chemistry, Spectrophotometry, Ultraviolet, Azides chemistry, Biotin analogs & derivatives, Nucleic Acids chemistry
Abstract

A new reagent, polyphotobiotin (PPhB), for labelling DNA probes has been obtained using low molecule oligoethylenimine (M 600 Da). Photoreactive group of PPhB is 4-azido-salicylic acid. The procedure is simple and quick, the reaction time being about 20 min. PPhB-labelled DNA has the same efficiency in the hybridisation analysis as DNA labelled with Bio-4-dUTP via PCR.

Published
1991

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