Search

Low-grade B cell non-Hodgkin's lymphoma with dominant presentation of chest wall mass is presented in this report. The patient, a 65-year-old woman, presented with pain, rising skin temperature and redness, and swelling on the right lower chest wall. The histopathological examination revealed non-Hodgkin's lymphoma; the staging fluorodeoxyglucose-positron emission tomography/computed tomography demonstrated stage IVE disease, with hypermetabolic active disease in the right anterolateral chest wall in the form of large soft tissue mass and subcutaneous tissue with underlying bony erosion with extension into right anterior cardiophrenic space and superiorly up to right second costosternal region along the right internal mammary vessels. This was along with hypermetabolic active right axillary, right supraclavicular and left inguinal lymphadenopathy, and thickened hypermetabolic posterior right pleura with ametabolic right-sided pleural effusion. Bone marrow biopsy revealed uninvolved bone marrow. On follow-up after eight cycles of R-CHOP chemotherapy, the mass had completely resolved on contrast-enhanced computed tomography. [ABSTRACT FROM AUTHOR]

Neoplastic transformation of germinal center B (GCB) cells may give rise to a variety of different B cell lymphoma subtypes, most of which show substantial heterogeneity in terms of genetic alterations and clinical features. The mutations observed in cancerrelated genes in GCB cells are related to abnormalities in the immunogenetic mechanisms associated with germinal center reaction. Recent studies have rapidly identified genomic alterations in B cell lymphomas that may be useful for better subclassification, noninvasive diagnosis, and prediction of response to therapy. The WHO recognizes different lymphoma subsets classified within 2 major categories of B cell lymphoma: Hodgkin's lymphoma (HL) and B cell non-Hodgkin's lymphoma (NHL), each with distinct genetic aberrations, including chromosomal translocations, copy number abnormalities, or point mutations. Next-generation sequencing-based technologies have allowed cancer researchers to identify somatic mutations and gene expression signatures at a rapid pace so that novel diagnostic or prognostic biomarkers, as well as therapeutic targets, can be discovered much faster than before. Indeed, deep sequencing studies have recently revealed that lymphoma-specific somatic mutations may be detected in cell-free circulating DNA obtained from the peripheral blood of B cell lymphoma patients, suggesting the possibility of minimally invasive diagnosis, monitoring, and predicting response to therapy of B cell lymphoma patients. In this study, the current status of the recurrent genetic aberrations observed during diagnosis and/ or relapse in HL and the major subtypes of B cell NHL (i.e. diffuse large B cell lymphoma, follicular lymphoma, mantle cell lymphoma, and Burkitt lymphoma) are discussed to shed light on their potential use as noninvasive diagnostic or prognostic biomarkers and to reveal their role in lymphomagenesis as a target in therapy for newly diagnosed and chemotherapy-resistant cases. [ABSTRACT FROM AUTHOR]

To exploit the B-lymphocyte antigen-CD20 binding capa­city of the Ibritumomab tiuxetan (IBTN) monoclonal antibody (mAb) for imaging, the over-expression of B cells in non-Hodgkin's lymphoma (NHL) (a myeloproliferative disorder of the lymphatic system) was investigated. In the current investigation, we present the labeling of the IBTN with technetium-99m (99mTc) through [99mTc(CO)3]+ precursor for radioimmunoimaging (RII) of the tumor prior to its treatment with 90Y labeled IBTN. Labeled IBTN was radiobiologically characterized in terms of radiochemical purity, in vitro stability in human plasma, immunoreactivity, binding with Raji and Ramos cells and biodistribution in a female nude mouse (FNM) model. It was observed that the reduced IBTN (rIBTN) showed more promising radiobiologic characteristics than the nonreduced IBTN. Significantly higher transchelation was seen in excess cysteine compared with histidine. The radioconjugate showed higher saturated binding affinity with CD20 antigen. Significantly higher target (tumor) to background ratios were observed 1 h post-injection (p.i.). Based on radiochemical purity, in vitro stability, immunoreactivity, binding and biodistrubtion in the FNM model, we recommend the radiolabeling of the rIBTN using tricarbonyl technique as a potential RII agent. [ABSTRACT FROM AUTHOR]

OBJECTIVES: B-cell Non-Hodgkin's lymphoma (B-NHL) is one of the increasing cancer species over the world. DICER1 gene is a member of ribonuclease type III family and it plays a crucial role in biogenesis of miRNAs that regulates the gene expression in posttranscriptional level. In addition, studies have showed that the germline mutations in DICER1 exon 11 may be hazardous because they result in frame shift and exon 25 mutations play role in biogenesis of miRNA. BAFF is expressed by immune system cells such as monocytes, macrophages, dendritic cells, a subtype of T-lymphocytes and B-lymphocytes. Studies, that has been done till now, show high levels BAFF gene expression in malignant B-cells of patients with B-NHL. MATERIAL&METHODS: In our study, DICER1 c.+3473A>G (rs3742330) and BAFF c.-871C>T (rs9514828) single nucleotide polymorphisms in DNA materials, that were isolated from peripheral blood samples of 60 patients that were diagnosed B-cell non-Hodgkin's lymphoma and 30 healthy people matched with patients by age, sex and ethnicity, were examined with PCR-RFLP method. RESULTS: The detection of mutation existence in exon11 and 25 in DICER1 gene was examined by SANGER sequencing analysis. Chi-Square and Fisher tests were used for evaluation of the results between control and patient groups. No significant results were found between the two groups (p > 0, 05). CONCLUSIONS: In our study, the presence of mutations and polymorphisms related to DICER1 and BAFF genes was not found in patients with B cell non- Hodgkin lymphoma. It is thought that the disease may be related to other factors in the patient group in our study. [ABSTRACT FROM AUTHOR]