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To develop environmentally safe multifunctional biological preparations, we carried out the primary screening of bacterial strains (from the collection of the biomethod laboratory of V.S. Pustovoit All-Russian Research Institute of Oil Crops) against the sunflower dry rot pathogen Rhizopus oryzae Went & Prins. Geerl. by the method of double or reciprocal cultures. 17 strains of the Bacillus genus exhibited antagonistic activity against the sunflower dry rot pathogen with two types of mechanism of action (competition for feeding area and antibiosis). Strains 01 kor f and D 1-1 Bacillus sp. competed for surface feeding area and inhibited the growth of aerial mycelium of the pathogen. We observed the maximum sterile zone in three strains: 3-3 Bacillus sp. and K 1-1 B. subtilis – 4.0 mm, and P-9 B. polymyxa – 3.0 mm. 6 bacterial strains of the genus Pseudomonas had antagonistic activity against the dry rot pathogen with one type of mechanism of action – antibiosis. Strain 12-2 Pseudomonas sp. had the largest sterile zone on potato sucrose agar (PSA) (5.5 mm), and strains 14-4 and 15-1 Pseudomonas sp. had the largest sterile zone on the King B medium (5.0 and 6.0 mm). [ABSTRACT FROM AUTHOR]

The article presents the results of development of technology elements of application of the laboratory samples of microbiopreparations in the preparative form of wettable powder (WP) based on the promising producer strains against the pathogen of oil flax Fusarium blight Fusarium oxysporum var. orthoceras on the background of artificial inoculation in a humidity chamber in vitro, depending on the application rate. On the backgrounds of flax inoculation with the Fusarium blight pathogen in control without treatment of 70.0 and 90.0 %, we established the optimum application rates for the laboratory samples of WP microbiopreparations: based on the fungal producer strain T-1 Trichoderma sp. with a titer of 2.3×1010 CFU/g – 0.05 kg/t, with the effectiveness of 71.4-57.2 %, respectively; based on the bacterial producer strain 11-3 Bacillus sp., with a titer of 5.5×1012 CFU/g – 0.3 kg/t, with the effectiveness of 100 and 85.7 %, respectively. [ABSTRACT FROM AUTHOR]

Compatibility of strains-producers of microbiopreparations with pesticides recommended for application on oil flax is determined. The strains-producers of microbiopreparations 11-3 Bacillus sp. and Т-1 Trichoderma sp. Are compatible with fungicides, insecticides, and a herbicide (TMTD, WSC (thiram, 400 g/l); Raxil Ultra, CS (tebuсonazole, 120 g/l); Faskord, EC (alpha-cypermethrin, 100 g/l); Rogor-S, EC (dimethoate, 400 g/l); Egida, SC (mesotrione, 480 g/l). This allows applying mixtures on their basis in an integrated crop protection. A partial compatibility of a fungus strain Т-1 Trichoderma sp. with a fungicide Raxil Ultra, CS is an exclusion. Bacterial and fungus strains-producers are not compatible with fungicides Redigo Pro, CS (prothioconazole, 150 g/l, tebuсonazole, 20 g/l) and Tebu 60, ME (tebuсonazole, 60 g/l). [ABSTRACT FROM AUTHOR]

The article presents the results of the development of technology elements of microbiopreparation laboratory samples application in the preparative form of wettable powder (WP) based on two promising producer strains against the pathogen of oil flax Fusarium blight Fusarium oxysporum var. orthoceras on the background of artificial inoculation in the soil depending on application rates. We established the optimum application rates for laboratory samples of WP on the background of oil flax inoculation with Fusarium blight pathogen of 67.5 % in control without treatment: based on fungal producer strain T-1 Trichoderma sp. – 0.05 kg/t; based on bacterial producer strain 11-3 Bacillus sp. – 0.3 kg/t. The laboratory sampleof WP based on the bacterial strain showed higher effectiveness against the pathogen of oil flax Fusarium blight. [ABSTRACT FROM AUTHOR]