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Enzymes are well known biocatalysts that execute a multitude of chemical reactions in the metabolism of almost all organisms. From an industrial perspective, only a restricted number of enzymes are commercially available and a few of them have found applications in large quantities. Microbial fibrinolytic enzymes are among the most important hydrolytic enzymes and have been studied extensively since the advent of enzyme technology. Alkaline fibrinolytic enzyme produced under solid state fermentation by Bacillus cereus GD55 was purified by ammonium sulphate fractionation (70%) and subjected to chromatographic methods. The purified enzyme produced 11.59 fold with recovery 6.8% by DEAE-Sephadex A-50 column chromatography and molecular weight was estimated to be 31 kDa by SDS-PAGE. This enzyme exhibited good compatibility with the commercial detergent Rin. The fibrinolytic potential of the enzyme was evidenced by the complete removal of blood stains from cotton cloth within 50 min of treatment. Bacillus cereus GD55 alkaline fibrinolytic enzyme with a diversity of activities, has great potential for application in a wide range of industry. [ABSTRACT FROM AUTHOR]

This work has been undertaken for the Screening and isolation of Keratinase producing strains of Bacteria were carried out from Ten soil samples, collected from various regions of Bangalore and used to screen for Keratinase production by using feather powder agar plate assay. In the present study, an attempt was made to isolate efficient Keratinase producing bacteria from diverse poultry waste. Different isolates were screened for possessing the ability to produce Keratinase. About 6 bacterial isolates were found to be promising to produce Keratinase. The organisms were tested for various biochemical tests, which leads to their identification as Bacillus licheniformis, Bacillus cerus, and Staphylococcus aureus. [ABSTRACT FROM AUTHOR]