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At high cell concentrations, bacterial suspensions are known to develop a state of collective swimming (the "zooming bionematic phase," or ZBN) characterized by transient, recurring regions of coordinated motion greatly exceeding the size of individual cells. Recent theoretical studies of semidilute suspensions have suggested that long-range hydrodynamic interactions between swimming cells are responsible for long-wavelength instabilities that lead to these patterns, while models appropriate for higher concentrations have suggested that steric interactions between elongated cells play an important role in the self-organization. Using particle imaging velocimetry in well-defined microgeometries, we examine the statistical properties of the transition to the ZBN in suspensions of Bacillus subtilis, with particular emphasis on the distribution of cell swimming speeds and its correlation with orientational order. This analysis reveals a nonmonotonic relationship between mean cell swimming speed and cell concentration, with a minimum occurring near the transition to the ZBN. Regions of high orientational order in the ZBN phase have locally high swimming speeds, while orientationally disordered regions have lower speeds. A model for steric interactions in concentrated suspensions and previous observations on the kinetics of flagellar rebundling associated with changes in swimming direction are used to explain this observation. The necessity of incorporating steric effects on cell swimming in theoretical models is emphasized.

Experiments and mathematical modeling show that complex flows driven by unexpected flagellar arrangements are induced when peritrichously flagellated bacteria are confined in a thin layer of fluid, between asymmetric boundaries. The flagella apparently form a dynamic bipolar assembly rather than the single bundle characteristic of free swimming bacteria, and the resulting flow is observed to circulate around the cell body. It ranges over several cell diameters, in contrast to the small extent of the flows surrounding free swimmers. Results also suggest that flagellar bundles on bacteria that lie flat on a solid substrate have an effective rotation rate slower than "free" flagella. This discovery extends our knowledge of the dynamic geometry of bacteria and their flagella, and reveals new mechanisms for motility-associated molecular transport and intercellular communication.