1. Levan production from sucrose using chicken feather peptone as a low cost supplemental nutrient source.
- Author
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Veerapandian B, Shanmugam SR, Varadhan S, Sarwareddy KK, Mani KP, and Ponnusami V
- Subjects
- Animals, Cell Survival, Chickens, Feathers chemistry, Fermentation, Fructans pharmacology, Human Umbilical Vein Endothelial Cells drug effects, Humans, Bacillus subtilis metabolism, Fructans metabolism, Peptones metabolism, Sucrose metabolism
- Abstract
Chicken feather peptone (CFP) derived from poultry waste is a rich source of essential minerals and amino acids. This, along with suitable carbon source, can be used as a low cost complex supplemental nutrient source for microbial fermentation. In the present work, CFP blended with sucrose was evaluated for the production of levan using Bacillus subtilis MTCC 441. Amount of CFP added to the medium significantly influenced levan production and it was found that at a concentration 2 g/L, maximum levan yield of 0.26 ± 0.04 g/g sucrose was obtained. The levan yield obtained with CFP as a low cost supplemental nutrient source was comparable with that obtained from commercial medium (0.31 ± 0.02 g/g sucrose). Levan produced using CFP was tested on primary cell lines at various concentrations (100-1000 μM) and found to be non-toxic and bio-compatible in nature. This indicates that CFP could be used as low cost nutrient source for levan production., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2020
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