1. Adherence and invasion of Bacteroidales isolated from the human intestinal tract
- Author
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Viviane Nakano, M.A. Menezes, V Szulczewski, Hannah M. Wexler, Mario Julio Avila-Campos, Vanessa Bueris, Roxane M. F. Piazza, Lilian Pumbwe, Aurora Marques Cianciarullo, Meire Roberta Bresciani Mendes-Ledesma, Waldir P. Elias, and Maurilio Fernandes dos Santos
- Subjects
Microbiology (medical) ,Diarrhea ,Virulence Factors ,capsule ,Fimbria ,Colony Count, Microbial ,Virulence ,medicine.disease_cause ,HEp-2 cells ,Bacterial Adhesion ,Microbiology ,Cell Line ,Feces ,fluids and secretions ,Cytosol ,medicine ,Bacteroides spp ,Animals ,Humans ,adherence ,Child ,Microscopy, Immunoelectron ,Bacteroidaceae ,Bacterial Capsules ,Parabacteroides spp ,biology ,Bacteroidetes ,food and beverages ,Infant ,Epithelial Cells ,General Medicine ,invasion ,biology.organism_classification ,Parabacteroides ,Bacteroidales ,Gastrointestinal Tract ,Microscopy, Electron ,Infectious Diseases ,Child, Preschool ,Fimbriae, Bacterial ,Parabacteroides distasonis ,Bacteroides ,Bacteria - Abstract
Members of the genera Bacteroides and Parabacteroides are important constituents of both human and animal intestinal microbiota, and are significant facultative pathogens. In this study, the ability of Bacteroides spp. and Parabacteroides distasonis isolated from both diarrhoeal and normal stools (n = 114) to adhere to and invade HEp-2 cells was evaluated. The presence of putative virulence factors such as capsule and fimbriae was also investigated. Adherence to HEp-2 cells was observed in 75.4% of the strains, which displayed non-localized clusters. Invasion was observed in 37.5% and 26% of the strains isolated from diarrhoeal and non-diarrhoeal stools, respectively. All strains displayed a capsule, whereas none of them showed fimbriae-like structures. This is the first report of the ability of Bacteroides spp. and P. distasonis to adhere to and invade cultured HEp-2 epithelial cells.
- Published
- 2008