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[Display omitted] • PLA/gel/ZnO nanofiber aerogel scaffolds with three-dimensional nanofibrous structure were developed. • PLA/gel/ZnO nanofiber aerogel scaffolds exhibited good antibacterial properties. • PLA/gel/ZnO nanofiber aerogel scaffolds could decrease inflammatory reactions and promote angiogenesis. • PLA/gel/ZnO nanofiber aerogel scaffolds significantly accelerated the wound healing process. Bacterial infection and severe wound inflammation are two primary harmful problems that bring harm to the human body and may cause death when a large-scale skin defect occurs. Thus, developing an effective and quick wound healing strategy for curing skin damage and trauma is vital. This study has developed a multifunctional PLA/gelatin/ZnO nanofiber aerogel with a three-dimensional structure through electrospinning and freeze-drying technology for wound healing. It has validated that the nanofiber aerogel has an excellent antibacterial property and biocompatibility. Meanwhile, benefiting from its three-dimensional nanofiber structure, the PLA/gelatin/ZnO nanofiber aerogel possesses good water absorption and air permeability. In vivo experiments have determined that the PLA/gel/ZnO nanofiber aerogel scaffolds effectively promote skin infection's wound healing and enhance angiogenesis that is practical with increasing ZnO concentration. [ABSTRACT FROM AUTHOR]

• Phage vB_ValP_IME234 is a novel virulent phage belonging to the podoviridae family, which can infect both V. alginolyticus and vibrio parahaemolyticus. • No integrase gene was detected, suggesting the phage is not temperate — which is important for application. • There were lysis genes and no antibiotic resistance or potential virulence genes in phage vB_ValP_IME234 genome, so this phage may serve as potential agents for phage therapy. Bacterial infections have a negative impact on both animal husbandry industry and medicine, and increasing bacterial drug resistance exacerbates this adverse impact. Phages show promise as an alternative to drugs against drug-resistant bacteria. In this study, a novel virulent bacteriophage (phage) vB_ValP_IME234 against Vibrio alginolyticus and Vibrio parahaemolyticus was isolated from freshwater in Beijing, China. Phage vB_ValP_IME234 had an isometric head (59 nm in diameter) and a short tail (10 nm long), belonging to Podoviridae family. Its complete genome is liner double-stranded DNA (dsDNA) with a GC content of 41.6% while encoding 61 putative proteins. Three transfer RNA (tRNA) and no lysogenic gene was detected. vB_ValP_IME234 had a polyvalent infectivity, a burst of 390 PFU/cell, and is stable under different temperatures (4 °C to 50 °C) and pH (6.0 to 10.0) values. Host range test showed that vB_ValP_IME234 has the ability to infect seven strains of Vibrio in total. Phylogenetic analyses based on terminase and capsid suggested that this phage had a close relationship with Vibrio phages. These results indicate that vB_ValP_IME234 could be used as a potential biocontrol agent against V. alginolyticus strains. [ABSTRACT FROM AUTHOR]