1. The sensitivity of the QuantiFERON®-TB Gold Plus assay in Zambian adults with active tuberculosis
- Author
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Telisinghe, L, Amofa-Sekyi, M, Maluzi, K, Kaluba-Milimo, D, Cheeba-Lengwe, M, Chiwele, K, Kosloff, B, Floyd, S, Bailey, S-L, and Ayles, H
- Subjects
latent tuberculous infection ,Adult ,Male ,validity ,Tuberculin Test ,Sputum ,Zambia ,HIV Infections ,Original Articles ,bacterial infections and mycoses ,Sensitivity and Specificity ,Logistic Models ,Latent Tuberculosis ,Humans ,Female ,Tuberculosis, Pulmonary ,Interferon-gamma Release Tests ,interferon-gamma release assays - Abstract
SUMMARY SETTING AND OBJECTIVE: To investigate the sensitivity of the new interferon-gamma release assay (IGRA), QuantiFERON®-TB Gold Plus (QFT-Plus), for active TB (used as a surrogate for latent tuberculous infection) in a Zambian TB clinic. DESIGN: Consecutive smear or Xpert® MTB/RIF-positive adult (age ⩾18 years) pulmonary TB patients were recruited between June 2015 and March 2016. Venous blood was tested using QFT-Plus. The sensitivity was defined as the number positive divided by the total number tested. Using logistic regression, factors associated with positive QFT-Plus results were explored. RESULTS: Of 108 patients (median age 32 years, interquartile range 27–38; 73% male; 63% human immunodeficiency virus [HIV] positive), 90 were QFT-Plus-positive, 11 were negative and seven had indeterminate results; sensitivity was 83% (95%CI 75–90). There was no difference in sensitivity by HIV status (HIV-positive 85%, 95%CI 75–93; n = 68 vs. HIV-negative 80%, 95%CI 64–91; n = 40; P = 0.59). In models adjusted for age alone, CD4 cell count
- Published
- 2017