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Your search keyword '"GDH positive/toxin negative"' showing total 2 results
Start Over Descriptor "GDH positive/toxin negative" Topic bacterial proteins
2 results on '"GDH positive/toxin negative"'

1. Evaluation of antigen-positive toxin-negative enzyme immunoassay results for the diagnosis of toxigenic Clostridium difficile infection.

Author

Akamatsu Y, Morishita S, Chikumi H, Okamoto R, Okada K, Kitaura T, Miyake N, Yamaguchi K, Nakamoto M, Shimohiro H, Takata M, Yamasaki A, Burioka N, and Shimizu E

Subjects
Aged, Female, Humans, Immunoenzyme Techniques, Male, Retrospective Studies, Antigens, Bacterial analysis, Bacterial Proteins analysis, Bacterial Toxins analysis, Clostridium Infections diagnosis, Enterotoxins analysis, Glutamate Dehydrogenase analysis
Abstract

Clostridium difficile (C. difficile)-associated diarrhea (CDAD) is a challenging nosocomial infectious disease. C. DIFF Quik Chek Complete assay is widely used to detect glutamate dehydrogenase (GDH) antigen and toxin A/B of C. difficile simultaneously. However, the interpretation of GDH positive/toxin negative results is problematic. We performed a retrospective study of patients with GDH positive/toxin negative results to determine the probability of detecting toxigenic C. difficile and its risk factors. Between April 2012 and March 2017, we investigated cultures of fecal specimens followed by toxin detection tests. The clinical histories of patients with and without toxigenic C. difficile were compared using univariate- and multivariate-analyses. In total, 2675 patients were examined using C. Diff Quik Chek Complete assay. Among 356 GDH positive/toxin negative patients, cultures were performed in 220 cases and toxigenic C. difficile was recovered from 139 (63.2%) specimens. Patients with toxigenic C. difficile had significantly lower body mass index than those without. Over half the GDH positive/toxin negative patients were infected with toxigenic C. difficile. Lower BMI was a CDAD risk factor in this patient population. These data can be utilized to initiate isolation and clinical interventions before confirmatory test results are available. J. Med. Invest. 65:131-135, February, 2018.

Published
2018
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2. Evaluation of antigen-positive toxin-negative enzyme immunoassay results for the diagnosis of toxigenic Clostridium difficile infection

Author

Masaki Nakamoto, Kensaku Okada, Yukinobu Akamatsu, Ryo Okamoto, Hiroki Chikumi, Tsuyoshi Kitaura, Naomi Miyake, Shota Morishita, Hisashi Shimohiro, Miyako Takata, Akira Yamasaki, Naoto Burioka, Eiji Shimizu, and Kosuke Yamaguchi

Subjects
0301 basic medicine, Male, 030106 microbiology, Bacterial Toxins, Clostridium difficile toxin A, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, Microbiology, Immunoenzyme Techniques, 03 medical and health sciences, Enterotoxins, Antigen, Bacterial Proteins, Glutamate Dehydrogenase, medicine, Humans, Clostridium difficile (C. difficile), Feces, GDH positive/toxin negative, Aged, Retrospective Studies, C. difficile-associated diarrhea (CDAD), Antigens, Bacterial, medicine.diagnostic_test, Toxin, business.industry, General Medicine, Clostridium difficile, Nosocomial infectious disease, Diarrhea, Immunoassay, Clostridium Infections, Female, C. Diff Quik Chek Complete assay, medicine.symptom, business
Abstract

Clostridium difficile (C. difficile)-associated diarrhea (CDAD) is a challenging nosocomial infectious disease. C. DIFF Quik Chek Complete assay is widely used to detect glutamate dehydrogenase (GDH) antigen and toxin A/B of C. difficile simultaneously. However, the interpretation of GDH positive/toxin negative results is problematic. We performed a retrospective study of patients with GDH positive/toxin negative results to determine the probability of detecting toxigenic C. difficile and its risk factors. Between April 2012 and March 2017, we investigated cultures of fecal specimens followed by toxin detection tests. The clinical histories of patients with and without toxigenic C. difficile were compared using univariate- and multivariate-analyses. In total, 2675 patients were examined using C. Diff Quik Chek Complete assay. Among 356 GDH positive/toxin negative patients, cultures were performed in 220 cases and toxigenic C. difficile was recovered from 139 (63.2%) specimens. Patients with toxigenic C. difficile had significantly lower body mass index than those without. Over half the GDH positive/toxin negative patients were infected with toxigenic C. difficile. Lower BMI was a CDAD risk factor in this patient population. These data can be utilized to initiate isolation and clinical interventions before confirmatory test results are available. J. Med. Invest. 65:131-135, February, 2018.

Published
2018

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