1. Use of Proteins Identified through a Functional Genomic Screen To Develop a Protein Subunit Vaccine That Provides Significant Protection against Virulent Streptococcus suis in Pigs.
- Author
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Brockmeier SL, Loving CL, Nicholson TL, Wang J, Peters SE, Weinert L, Chaudhuri R, Seilly DJ, Langford PR, Rycroft A, Wren BW, Maskell DJ, and Tucker AW
- Subjects
- Animals, Antibodies, Bacterial immunology, Bacterial Proteins administration & dosage, Bacterial Proteins genetics, Cross Protection, Female, Genomics, Male, Streptococcal Infections immunology, Streptococcal Infections microbiology, Streptococcal Infections prevention & control, Streptococcal Vaccines genetics, Streptococcal Vaccines immunology, Streptococcus suis chemistry, Streptococcus suis genetics, Streptococcus suis pathogenicity, Swine, Swine Diseases immunology, Swine Diseases microbiology, Vaccines, Subunit administration & dosage, Vaccines, Subunit genetics, Vaccines, Subunit immunology, Virulence, Bacterial Proteins immunology, Streptococcal Infections veterinary, Streptococcal Vaccines administration & dosage, Streptococcus suis immunology, Swine Diseases prevention & control
- Abstract
Streptococcus suis is a bacterium that is commonly carried in the respiratory tract and that is also one of the most important invasive pathogens of swine, commonly causing meningitis, arthritis, and septicemia. Due to the existence of many serotypes and a wide range of immune evasion capabilities, efficacious vaccines are not readily available. The selection of S. suis protein candidates for inclusion in a vaccine was accomplished by identifying fitness genes through a functional genomics screen and selecting conserved predicted surface-associated proteins. Five candidate proteins were selected for evaluation in a vaccine trial and administered both intranasally and intramuscularly with one of two different adjuvant formulations. Clinical protection was evaluated by subsequent intranasal challenge with virulent S. suis While subunit vaccination with the S. suis proteins induced IgG antibodies to each individual protein and a cellular immune response to the pool of proteins and provided substantial protection from challenge with virulent S. suis , the immune response elicited and the degree of protection were dependent on the parenteral adjuvant given. Subunit vaccination induced IgG reactive against different S. suis serotypes, indicating a potential for cross protection., (Copyright © 2018 American Society for Microbiology.)
- Published
- 2018
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