1. A second operator is involved in Pseudomonas aeruginosa elastase (lasB) activation.
- Author
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Anderson RM, Zimprich CA, and Rust L
- Subjects
- Base Sequence, Enzyme Activation, Enzyme Induction, Molecular Sequence Data, Mutagenesis, Site-Directed, Pseudomonas aeruginosa enzymology, RNA, Bacterial biosynthesis, RNA, Messenger biosynthesis, Bacterial Proteins, Gene Expression Regulation, Bacterial, Metalloendopeptidases biosynthesis, Operator Regions, Genetic, Pseudomonas aeruginosa genetics
- Abstract
Pseudomonas aeruginosa LasB elastase gene (lasB) transcription is controlled by the two-component quorum-sensing system of LasR, and the autoinducer, 3OC(12)-HSL (N-3-[oxododecanoyl]homoserine lactone). LasR and 3OC(12)-HSL-mediated lasB activation requires a functional operator sequence (OP1) in the lasB promoter region. Optimal activation of lasB, however, requires a second sequence of 70% identity to OP1, named OP2, located 43 bp upstream of OP1. In this study, we used sequence substitutions and insertion mutations in lasBp-lacZ fusion plasmids to explore the role of OP2 in lasB activation. Our results demonstrate that (i) OP1 and OP2 synergistically mediate lasB activation; (ii) OP2, like OP1, responds to LasR and 3OC(12)-HSL; and (iii) the putative autoinducer-binding domain of LasR is not required for synergistic activation from OP1 and OP2.
- Published
- 1999
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