Search

Your search keyword '"Priputnevich TV"' showing total 2 results
Start Over Author "Priputnevich TV" Topic bacterial typing techniques
2 results on '"Priputnevich TV"'

1. Method of Selection of Bacteria Antibiotic Resistance Genes Based on Clustering of Similar Nucleotide Sequences.

Author

Balashov IS, Naumov VA, Borovikov PI, Gordeev AB, Dubodelov DV, Lyubasovskaya LA, Rodchenko YV, Bystritskii AA, Aleksandrova NV, Trofimov DY, and Priputnevich TV

Subjects
Aminoglycosides pharmacology, DNA Primers genetics, Enterococcus faecalis drug effects, Enterococcus faecalis genetics, Enterococcus faecalis growth & development, Escherichia coli drug effects, Escherichia coli genetics, Escherichia coli growth & development, Fluoroquinolones pharmacology, Fusidic Acid pharmacology, Gardnerella vaginalis drug effects, Gardnerella vaginalis genetics, Gardnerella vaginalis growth & development, Glycopeptides pharmacology, Klebsiella pneumoniae drug effects, Klebsiella pneumoniae genetics, Klebsiella pneumoniae growth & development, Lincosamides pharmacology, Macrolides pharmacology, Microbial Sensitivity Tests, Multigene Family, Staphylococcus drug effects, Staphylococcus genetics, Staphylococcus growth & development, Streptococcus agalactiae drug effects, Streptococcus agalactiae genetics, Streptococcus agalactiae growth & development, beta-Lactams pharmacology, Anti-Bacterial Agents pharmacology, Bacterial Typing Techniques methods, DNA Primers chemical synthesis, DNA, Bacterial genetics, Drug Resistance, Multiple, Bacterial genetics, Polymerase Chain Reaction methods
Abstract

A new method for selection of bacterium antibiotic resistance genes is proposed and tested for solving the problems related to selection of primers for PCR assay. The method implies clustering of similar nucleotide sequences and selection of group primers for all genes of each cluster. Clustering of resistance genes for six groups of antibiotics (aminoglycosides, β-lactams, fluoroquinolones, glycopeptides, macrolides and lincosamides, and fusidic acid) was performed. The method was tested for 81 strains of bacteria of different genera isolated from patients (K. pneumoniae, Staphylococcus spp., S. agalactiae, E. faecalis, E. coli, and G. vaginalis). The results obtained by us are comparable to those in the selection of individual genes; this allows reducing the number of primers necessary for maximum coverage of the known antibiotic resistance genes during PCR analysis.

Published
2017
Full Text
View/download PDF

2. [MASS-SPECTROMETRY IN MICROBIOLOGICAL PRACTICE OF SCIENTIFIC CENTRE OF OBSTETRICS, GYNECOLOGY AND PERINATOLOGY].

Author

Priputnevich TV, Melkumyan AR, Lyubasovskaya LA, Muravieva VV, Ilina EN, and Sukhikh GT

Subjects
Bacteria genetics, Bacteria isolation & purification, Female, Fungi genetics, Fungi isolation & purification, Gynecology, Humans, Infant, Newborn, Obstetrics, Perinatology, Pregnancy, RNA, Ribosomal isolation & purification, Sequence Analysis, RNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Vagina microbiology, Academic Medical Centers methods, Bacteria classification, Bacterial Typing Techniques instrumentation, Fungi classification, Mycological Typing Techniques instrumentation, RNA, Ribosomal genetics
Abstract

Aim: Comparative evaluation of species identification of microorganisms by MALDI-TOF mass-spectrometry and automatic biochemical analyzer VITEK2 Compact 30., Materials and Methods: Species identification of 18,400 isolates of microorganisms (staphylococci, streptococci, enterococci, enterobacteria, nonfermenting gram-negative bacteria, lactobacilli, anaerobes, yeast fungi, neisseriae), isolated from vagina of pregnant and non-pregnant women and from newborns, was carried out. Identification of the isolated microorganisms was carried out by automatic bacteriologic analyzer VITEK2 Compact30 (BioMerieuX, France) and MALDI-TOF-MS analysis method on AutofleXIII (Bruker Daltonics, Germany) mass-spectrometer., Results: Comparative identification of 2005 isolates of microorganisms was carried out. Sequencing of ribosomal RNA was used as a reference method. Authenticity of species identification my MALDI-TOF-MS analysis method was: for staphylococci (95.8%), enterococci (97.5%), enterobacteria (98.4%), nonfermenting gram-negative bacteria (93.6%), β-hemolytiC staphylococci (93.8%), lactobacilli (92.8%), yeast fungi (99.9%)., Conclusion: Introduction of MALDI-TOF-MS analysis technology into practical work of microbiological laboratories exceeds previously used methods of microbiological testing in terms of speed; cost and authenticity of identification of a wide spectrum of microorganisms.

Published
2016

Catalog

Books, media, physical & digital resources
See catalog results