1. In vitro toxicity testing for antibacterials against human keratinocytes.
- Author
-
Smoot EC 3rd, Kucan JO, Roth A, Mody N, and Debs N
- Subjects
- Anti-Bacterial Agents pharmacology, Cell Division drug effects, Cell Movement drug effects, Cell Survival drug effects, Cells, Cultured, Flow Cytometry, Humans, Keratinocytes physiology, Mafenide pharmacology, Nitrofurazone pharmacology, Povidone-Iodine pharmacology, Silver Nitrate pharmacology, Silver Sulfadiazine pharmacology, Sodium Chloride pharmacology, Sodium Hypochlorite pharmacology, Anti-Infective Agents, Local pharmacology, Bacteriological Techniques, Keratinocytes drug effects
- Abstract
The use of cultured human keratinocytes in an in vitro comparison of topical antibacterial toxicity for epithelial cells was examined. The complement of three assessments allows testing of epithelial migration, growth, and survival. The three assessments included (1) flow cytometry for determination of cell survival, (2) a comparison of confluent cell culture growth after antibacterial exposures, and (3) an evaluation of cell migration using a technique of dermal explants to study radial migration. A comparative ranking of the toxicities of the various topical antibacterials was determined with the three assessments. This has confirmed anecdotal reports that many of the topical antibacterials are cell-toxic and may inhibit wound healing. This information can be directly extrapolated to the clinical setting, unlike many of the animal data for wound healing that currently exist.
- Published
- 1991
- Full Text
- View/download PDF