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Your search keyword '"Saatkamp, Helmut W."' showing total 3 results
Start Over Author "Saatkamp, Helmut W." Topic bacteriology
3 results on '"Saatkamp, Helmut W."'

1. Genomic evolution of antimicrobial resistance in Escherichia coli

Author

Leekitcharoenphon, Pimlapas, Johansson, Markus Hans Kristofer, Munk, Patrick, Malorny, Burkhard, Skarzynska, Magdalena, Wadepohl, Katharina, Moyano, Gabriel, Hesp, Ayla, Veldman, Kees T., Bossers, Alex, Graveland, Haitske, van Essen, Alieda, Battisti, Antonio, Caprioli, Andrea, Blaha, Thomas, Hald, Tine, Daskalov, Hristo, Saatkamp, Helmut W., Staerk, Katharina D. C., Luiken, Roosmarijn E. C., Van Gompel, Liese, Hansen, Rasmus Borup, Dewulf, Jeroen, Duarte, Ana Sofia Ribeiro, Zajac, Magdalena, Wasyl, Dariusz, Sanders, Pascal, Gonzalez-Zorn, Bruno, Brouwer, Michael S. M., Wagenaar, Jaap A., Heederik, Dick J. J., Mevius, Dik, Aarestrup, Frank M., EFFORT Consortium, for the, Technical University of Denmark [Lyngby] (DTU), Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment (BfR), Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Wageningen BioVeterinary Research, Wageningen University and Research [Wageningen] (WUR), Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), European Project: 613754,EC:FP7:KBBE,FP7-KBBE-2013-7-single-stage,EFFORT(2013), Danmarks Tekniske Universitet = Technical University of Denmark (DTU), IRAS OH Epidemiology Microbial Agents, Klinische infectiologie en microb. lab., dI&I I&I-4, dIRAS RA-I&I RA, and Faculteit Diergeneeskunde

Subjects
0301 basic medicine, Genomic alteration, antibiotic resistance, Swine, Epidemiology, résistance aux antibiotiques, veterinary drug, medicine.disease_cause, Genome, Poultry, Feces, animal, évolution, bacteria, Escherichia coli Infections, Phylogeny, médicament humainélevage, 2. Zero hunger, Genetics, human drug, bactérie, Multidisciplinary, Virulence, Bacteriologie, transmission, Genomics, Bacteriology, Host Pathogen Interaction & Diagnostics, Text, Anti-Bacterial Agents, Europe, Medicine, médicament, médicament vétérinaire, Bioinformatica & Diermodellen, Science, 030106 microbiology, Microbial Sensitivity Tests, GENETIC-STRUCTURE, Biology, Article, Evolution, Molecular, 03 medical and health sciences, Antibiotic resistance, résistance aux antimicrobiens, Drug Resistance, Bacterial, Bio-informatics & Animal models, medicine, Escherichia coli, Animals, Life Science, Epidemiology, Bio-informatics & Animal models, ddc:610, Veterinary Sciences, antimicrobial resistance, microbiologie, General, genome, Host Pathogen Interaction & Diagnostics, Epidemiologie, Whole genome sequencing, génome, microbiology, Bacteriology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Host Pathogen Interactie & Diagnostiek, livestock, Red Meat, 030104 developmental biology, Metagenomics, Genetic marker, Epidemiologie, Bioinformatica & Diermodellen, Bacteriologie, Host Pathogen Interactie & Diagnostiek, Molecular evolution, Cattle, POULTRY, Mobile genetic elements, humain, Genome, Bacterial
Abstract

The emergence of antimicrobial resistance (AMR) is one of the biggest health threats globally. In addition, the use of antimicrobial drugs in humans and livestock is considered an important driver of antimicrobial resistance. The commensal microbiota, and especially the intestinal microbiota, has been shown to have an important role in the emergence of AMR. Mobile genetic elements (MGEs) also play a central role in facilitating the acquisition and spread of AMR genes. We isolated Escherichia coli (n = 627) from fecal samples in respectively 25 poultry, 28 swine, and 15 veal calf herds from 6 European countries to investigate the phylogeny of E. coli at country, animal host and farm levels. Furthermore, we examine the evolution of AMR in E. coli genomes including an association with virulence genes, plasmids and MGEs. We compared the abundance metrics retrieved from metagenomic sequencing and whole genome sequenced of E. coli isolates from the same fecal samples and farms. The E. coli isolates in this study indicated no clonality or clustering based on country of origin and genetic markers; AMR, and MGEs. Nonetheless, mobile genetic elements play a role in the acquisition of AMR and virulence genes. Additionally, an abundance of AMR was agreeable between metagenomic and whole genome sequencing analysis for several AMR classes in poultry fecal samples suggesting that metagenomics could be used as an indicator for surveillance of AMR in E. coli isolates and vice versa.

Published
2021
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2. Abundance and diversity of the fecal resistome in slaughter pigs and broilers in nine European countries

Author

Munk, Patrick, Knudsen, Berith Elkær, Lukjacenko, Oksana, Duarte, Ana Sofia Ribeiro, Van Gompel, Liese, Luiken, Roosmarijn E.C., Smit, Lidwien A.M., Schmitt, Heike, Garcia, Alejandro Dorado, Hansen, Rasmus Borup, Petersen, Thomas Nordahl, Bossers, Alex, Ruppé, Etienne, Graveland, Haitske, van Essen, Alieda, Gonzalez-Zorn, Bruno, Moyano, Gabriel, Sanders, Pascal, Chauvin, Claire, David, Julie, Battisti, Antonio, Caprioli, Andrea, Dewulf, Jeroen, Blaha, Thomas, Wadepohl, Katharina, Brandt, Maximiliane, Wasyl, Dariusz, Skarzyńska, Magdalena, Zajac, Magdalena, Daskalov, Hristo, Saatkamp, Helmut W., Stärk, Katharina D.C., Lund, Ole, Hald, Tine, Pamp, Sünje Johanna, Vigre, Håkan, Heederik, Dick, Wagenaar, Jaap A., Mevius, Dik, Aarestrup, Frank M., One Health Microbieel, LS Klinisch Onderzoek Wagenaar, Dep IRAS, dIRAS RA-I&I RA, dIRAS RA-I&I I&I, dI&I I&I-4, Institute for Risk Assessment Sciences [Utrecht, The Netherlands] (IRAS), Utrecht University [Utrecht], Part of Wageningen UR, Central Veterinary Institute, Genomic Research Laboratory [Geneva, Switzerland], Geneva University Hospital (HUG), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratoire de Ploufragan - Plouzané, Center for Biological Sequence Analysis, Technical University of Denmark [Lyngby] (DTU), Division of Occupational and Environmental Health, Institute for Risk Assessment (IRAS), EFFORT Group, One Health Microbieel, LS Klinisch Onderzoek Wagenaar, Dep IRAS, dIRAS RA-I&I RA, dIRAS RA-I&I I&I, and dI&I I&I-4

Subjects
0301 basic medicine, pig, Veterinary medicine, antibiotic resistance, Epidemiology, Swine, WASS, Applied Microbiology and Biotechnology, Feces, Environmental DNA, animal, proc, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, bactérie, 0303 health sciences, poultry, Bacteriologie, Bacteriology, Host Pathogen Interaction & Diagnostics, Biodiversity, Poultry farming, Human morbidity, Europe, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Livestock, Gene pool, Microbiology (medical), Bioinformatica & Diermodellen, Immunology, Bedrijfseconomie, Coronacrisis-Taverne, Biology, 03 medical and health sciences, Antibiotic resistance, SDG 3 - Good Health and Well-being, Bacterial Proteins, Species Specificity, Business Economics, Bio-informatics & Animal models, Drug Resistance, Bacterial, Genetics, Life Science, Animals, Epidemiology, Bio-informatics & Animal models, microbiologie, 030304 developmental biology, Host Pathogen Interaction & Diagnostics, Epidemiologie, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Bacteria, 030306 microbiology, business.industry, résistane aux antibiotiques, Gene Expression Profiling, microbiology, Bacteriology, Cell Biology, Sequence Analysis, DNA, Host Pathogen Interactie & Diagnostiek, Biotechnology, Resistome, volaille, 030104 developmental biology, Metagenomics, Epidemiologie, Bioinformatica & Diermodellen, Bacteriologie, Host Pathogen Interactie & Diagnostiek, business, Chickens
Abstract

EFFORT groupHaitske Graveland, Alieda van Essen, Bruno Gonzalez-Zorn, Gabriel Moyano, Pascal Sanders, Claire Chauvin, Julie David, Antonio Battisti, Andrea Caprioli, Jeroen Dewulf, Thomas Blaha, Katharina Wadepohl, Maximiliane Brandt, Dariusz Wasyl, Magdalena Skarzyñska, Magdalena Zajac, Hristo Daskalov, Helmut W Saatkamp, Katharina D.C. Stärk.AbstractAntimicrobial resistance (AMR) in bacteria and associated human morbidity and mortality is increasing. Use of antimicrobials in livestock selects for AMR that can subsequently be transferred to humans. This flow of AMR between reservoirs demands surveillance in livestock as well as in humans. As part of the EFFORT project (www.effort-against-amr.eu), we have quantified and characterized the acquired resistance gene pools (resistomes) of 181 pig and 178 poultry farms from nine European countries, generating more than 5,000 gigabases of DNA sequence, using shotgun metagenomics. We quantified acquired AMR using the ResFinder database and a database constructed for this study, consisting of AMR genes identified through screening environmental DNA. The pig and poultry resistomes were very different in abundance and composition. There was a significant country effect on the resistomes, more so in pigs than poultry. We found higher AMR loads in pigs, while poultry resistomes were more diverse. We detected several recently described, critical AMR genes, includingmcr-1andoptrA, the abundance of which differed both between host species and countries. We found that the total acquired AMR level, was associated with the overall country-specific antimicrobial usage in livestock and that countries with comparable usage patterns had similar resistomes. Novel, functionally-determined AMR genes were, however, not associated with total drug use.

Published
2017
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3. Author Correction: Abundance and diversity of the faecal resistome in slaughter pigs and broilers in nine European countries

Author

Munk, Patrick, Knudsen, Berith Elkær, Lukjancenko, Oksana, Duarte, Ana Sofia Ribeiro, Van Gompel, Liese, Luiken, Roosmarijn E.C., Smit, Lidwien A.M., Schmitt, Heike, Garcia, Alejandro Dorado, Hansen, Rasmus Borup, Petersen, Thomas Nordahl, Bossers, Alex, Ruppé, Etienne, Lund, Ole, Hald, Tine, Pamp, Sünje Johanna, Vigre, Håkan, Heederik, Dick, Wagenaar, Jaap A., Mevius, Dik, Aarestrup, Frank M., Graveland, Haitske, van Essen, Alieda, Gonzalez-Zorn, Bruno, Moyano, Gabriel, Sanders, Pascal, Chauvin, Claire, David, Julie, Battisti, Antonio, Caprioli, Andrea, Dewulf, Jeroen, Blaha, Thomas, Wadepohl, Katharina, Brandt, Maximiliane, Wasyl, Dariusz, Skarzyńska, Magdalena, Zajac, Magdalena, Daskalov, Hristo, Saatkamp, Helmut W., Stärk, Katharina D.C., Institute for Risk Assessment Sciences [Utrecht, The Netherlands] (IRAS), Utrecht University [Utrecht], Part of Wageningen UR, Central Veterinary Institute, Genomic Research Laboratory [Geneva, Switzerland], Geneva University Hospital (HUG), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratoire de Ploufragan - Plouzané, and EFFORT Group

Subjects
pig, 0301 basic medicine, Microbiology (medical), antibiotic resistance, Epidemiology, Bioinformatica & Diermodellen, media_common.quotation_subject, Bedrijfseconomie, Immunology, Zoology, WASS, résistance aux antibiotiques, Biology, Applied Microbiology and Biotechnology, Microbiology, 03 medical and health sciences, Antibiotic resistance, Microbial ecology, Business Economics, Abundance (ecology), Bio-informatics & Animal models, Genetics, Life Science, Epidemiology, Bio-informatics & Animal models, animal, microbiologie, bacteria, ComputingMilieux_MISCELLANEOUS, media_common, Host Pathogen Interaction & Diagnostics, Epidemiologie, bactérie, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, poultry, Bacteriologie, Bacteriology, Bacteriology, Host Pathogen Interaction & Diagnostics, Cell Biology, Host Pathogen Interactie & Diagnostiek, Resistome, volaille, Europe, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, 030104 developmental biology, feces, Epidemiologie, Bioinformatica & Diermodellen, Bacteriologie, Host Pathogen Interactie & Diagnostiek, porc, Diversity (politics)
Abstract

In the version of this Article originally published, the surname of author Oksana Lukjancenko was spelt incorrectly as ‘Lukjacenko’. This has now been corrected.

Published
2018
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