1. Protein-protein interactions in a higher-order structure direct lambda site-specific recombination.
- Author
-
Thompson JF, de Vargas LM, Skinner SE, and Landy A
- Subjects
- Allosteric Site, Bacterial Proteins metabolism, Binding Sites, DNA-Binding Proteins metabolism, Integration Host Factors, Mutation, Viral Core Proteins metabolism, Bacteriophage lambda genetics, DNA, Recombinant metabolism, DNA, Viral metabolism, Viral Proteins metabolism
- Abstract
The highly directional site-specific recombination of bacteriophage lambda is tightly regulated by the binding of three different proteins to a complex array of sites. The manner in which these reactions are both stimulated and inhibited by co-operative binding of proteins to specific sites on the P arm of attP and AttR has been elucidated by correlation of nuclease protection with recombination studies of both wild-type and mutant DNAs. In addition to co-operative forces, there is a specific competitive interaction that allows the protein-DNA complex to serve as a "biological switch". This switch does not depend upon the simple occlusion of DNA binding sites by neighboring proteins; but, rather, the outcome of this competition is dependent on long-range interactions that vary between the higher-order structures of attP and attR. These higher-order structures are dependent on cooperative interactions involving three proteins binding to five or more sites.
- Published
- 1987
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