1. Synergistic phage-surfactant combination clears IgE-promoted Staphylococcus aureus aggregation in vitro and enhances the effect in vivo.
- Author
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Kim SG, Giri SS, Yun S, Kim HJ, Kim SW, Kang JW, Han SJ, Kwon J, Oh WT, Jun JW, and Park SC
- Subjects
- Animals, Dermatitis, Atopic microbiology, Humans, Immunoglobulin E immunology, Interferon-gamma biosynthesis, Interleukin-12 biosynthesis, Interleukin-1beta biosynthesis, Mice, Mice, Inbred BALB C, Staphylococcus aureus drug effects, Staphylococcus aureus immunology, Staphylococcus aureus virology, Bacterial Adhesion drug effects, Bacteriophages metabolism, Dermatitis, Atopic drug therapy, Phage Therapy methods, Staphylococcal Skin Infections drug therapy, Surface-Active Agents pharmacology
- Abstract
Currently, topical antibiotic treatment is a major strategy for decolonisation of Staphylococcus aureus, although it may result in antibiotic resistance or recolonisation of the organism. Recently, application of bacteriophages in the treatment of S. aureus infection has attracted attention. However, a single administration of bacteriophages did not effectively decolonise S. aureus in our first trial in vivo. Using a bacteriophage (pSa-3) and surfactant combination in vitro, we showed an increased (>8%) adsorption rate of the bacteriophage on the host. Moreover, the combination increased the eradication of immunoglobulin E (IgE)-stimulated aggregation, as the surfactant promoted the dissociation of S. aureus aggregates by decreasing the size by 75% and 50% in the absence and presence of IgE, respectively. Furthermore, the combined treatment significantly decolonised the pathogen with an efficacy double that of the phage-only treatment, and decreased the expression of pro-inflammatory cytokine genes (IL-1β, IL-12 and IFNγ) for 5 days in the second in vivo trial. These results suggest that the bacteriophage-surfactant combination could act as an alternative to antibiotics for S. aureus decolonisation in patients with dermatitis., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2020
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