1. Mechanistic insight into the synthesis of fucooligosaccharides by α-L-fucosidase from Thermotoga maritima belonging to the GH29 family: in silico study.
- Author
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Pavón-Chimal, Mauricio E., Jiménez-Pérez, Carlos, Guzmán-Rodriguez, Francisco, Alatorre-Santamaría, Sergio, González-Olivares, Luis G., García-Garibay, Mariano, Gómez-Ruiz, Lorena, Rodríguez-Serrano, Gabriela, and Cruz-Guerrero, Alma E.
- Subjects
THERMOTOGA maritima ,BREAST milk ,BANKING industry ,MOLECULAR docking ,OLIGOSACCHARIDES ,LACTOSE - Abstract
In recent years, the synthesis of human milk oligosaccharides, particularly fucooligosaccharides, has been investigated. These oligosaccharides provide protection against gastrointestinal diseases for newborns, among other functionalities. α-L-fucosidase from Thermotoga maritima of GH29 family has been reported to produce fucooligosaccharides by means of a transfucosylation reaction. However, the interaction between acceptor and donor substrates, and the enzyme has been little studied. For that reason, and due to the importance of fucooligosaccharides, the present study proposes a theoretical synthesis route employing α-L-fucosidase from T. maritima, lactose as acceptor substrate and pNP-fucose as fucosyl donor. A molecular docking study was carried out using crystallographic structure of α-L-fucosidase from Protein Data Bank. By simulating the theoretical synthesis with T. maritima fucosidase, it was observed that lactose interacts with the enzyme in regions other than the active site. However, it is approaching the active site when the fucose is in the position to form the enzyme-substrate complex. This finding may explain the transfucosylation yield documented in the literature when the proportion of fucosyl acceptor substrate increases concerning that of the donor substrate. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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