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Abstract: The montado is an agro-silvo pastoral system characterized by an open oak formation combined with shrubs or a rotation of cultures/pastures/fallow. A key, but frequently overlooked, component of these systems is the fungal community associated with the dominant oaks. We present a study of the basidiomycete community in montado areas of Quercus suber L. from Central-Western Portugal, based on fruiting body assessment and PCR-DGGE of soil mycelium. Soil available phosphorus and organic matter content were also determined. The most frequent fruiting body found in the cork oak montados belonged to Lactarius, Clitocybe, Russula and Cortinarius species. Lactarius chrysorrheus was the most widely distributed species. Soil management practices cause a temporal increase in soil available phosphorus, and harrowing and fertilizer application also result in an increase in soil organic matter content. Mechanical clearing with recent soil disturbance had a negative effect on the richness and diversity of the basidiomycete community assessed using fruiting body. The molecular analysis of the basidiomycete community separated the sites with recent shrub-removal from the others sites. Our results show that soil management techniques that avoid disturbance of the top-soil layers are the best way to preserve the structure and diversity of the soil fungal community in the montado. [ABSTRACT FROM AUTHOR]