1. Derivatives of the cationic plant alkaloids berberine and palmatine amplify protonophorous activity of fatty acids in model membranes and mitochondria.
- Author
-
Pustovidko AV, Rokitskaya TI, Severina II, Simonyan RA, Trendeleva TA, Lyamzaev KG, Antonenko YN, Rogov AG, Zvyagilskaya RA, Skulachev VP, and Chernyak BV
- Subjects
- Alkaloids metabolism, Cations metabolism, Hydrogen metabolism, Membranes drug effects, Mitochondria drug effects, Plastoquinone metabolism, Berberine metabolism, Berberine Alkaloids metabolism, Fatty Acids metabolism, Membranes metabolism, Mitochondria metabolism
- Abstract
Previously it has been shown by our group that berberine and palmatine, penetrating cations of plant origin, when conjugated with plastoquinone (SkQBerb and SkQPalm), can accumulate in isolated mitochondria or in mitochondria of living cells and effectively protect them from oxidative damage. In the present work, we demonstrate that SkQBerb, SkQPalm, and their analogs lacking the plastoquinone moiety (C10Berb and C10Palm) operate as mitochondria-targeted compounds facilitating protonophorous effect of free fatty acids. These compounds induce proton transport mediated by small concentrations of added fatty acids both in planar and liposomal model lipid membranes. In mitochondria, such an effect can be carried out by endogenous fatty acids and the adenine nucleotide translocase., (Copyright © 2012 Elsevier B.V. and Mitochondria Research Society. All rights reserved. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF