1. Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation.
- Author
-
Dehelean, Cristina A., Şoica, Codruţa, Ledeţi, Ionuţ, Aluaş, Mihaela, Zupko, Istvan, Găluşcan, Atena, Cinta-Pinzaru, Simona, and Munteanu, Melania
- Subjects
- *
BETULIN , *TRITERPENES , *BIRCH , *CANCER cells , *SPECTRUM analysis , *SPECTROMETRY - Abstract
Background: Pentacyclic triterpenes, mainly betulin and betulinic acid, are valuable anticancer agents found in the bark of birch tree. This study evaluates birch bark extracts for the active principles composition. Results: New improved extraction methods were applied on the bark of Betula pendula in order to reach the maximum content in active principles. Extracts were analyzed by HPLC-MS, Raman, SERS and 13C NMR spectroscopy which revealed a very high yield of betulin (over 90%). Growth inhibiting effects were measured in vitro on four malignant human cell lines: A431 (skin epidermoid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma) and MCF7 (breast adenocarcinoma), by means of MTT assay. All of the prepared bark extracts exerted a pronounced antiproliferative effect against human cancer cell lines. In vivo studies involved the anti-inflammatory effect of birch extracts on TPA-induced model of inflammation in mice. Conclusions: The research revealed the efficacy of the extraction procedures as well as the antiproliferative and anti-inflammatory effects of birch extracts. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF