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Mandibular second premolars and maxillary central incisors usually have one root, one canal, and one apical foramen. However, some studies have revealed anatomic variations in these teeth. The aim of the present study was to investigate such variations in canal configuration, foramina, lateral and accessory canals and apical deltas in the root apex of human maxillary central incisors and mandibular second premolars, using a clearing technique and stereomicroscopy. One hundred maxillary central incisors and 137 mandibular second premolars were collected, and India ink was injected into their canals. The teeth were then demineralized with 0.5 N nitric acid, cleared with methyl salicylate, and studied at×5 and × 16 magnification. The incidence of one canal and one apical foramen was 100% for maxillary central incisors and 94.16% for mandibular second premolars. The main apical foramen was located in the center of the root apex in 21.89% and 17% of mandibular second premolars and maxillary central incisors, respectively. Lateral and accessory canals were found in 84.50% and 77.15% of maxillary central incisors and mandibular second premolars, respectively. Several foramina were found in 11% of maxillary central incisors and 24.08% of mandibular second premolars. Apical deltas were seen in 4.38% and 2% of mandibular second premolars and maxillary central incisors, respectively. The rate of anatomic variations in the apical part of the tooth, especially in posterior teeth, is thus considered to be high. [ABSTRACT FROM AUTHOR]