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1. The development of allergic inflammation in a murine house dust mite asthma model is suppressed by synbiotic mixtures of non-digestible oligosaccharides and Bifidobacterium breve M-16V.

Author

Verheijden KA, Willemsen LE, Braber S, Leusink-Muis T, Jeurink PV, Garssen J, Kraneveld AD, and Folkerts G

Subjects
Animals, Asthma immunology, Bronchoalveolar Lavage Fluid microbiology, Chemokine CCL17 metabolism, Chemokine CCL22 metabolism, Disease Models, Animal, Eosinophils metabolism, Hypersensitivity immunology, Inflammation immunology, Interferon-gamma metabolism, Interleukins metabolism, Lung cytology, Lung metabolism, Male, Mice, Mice, Inbred BALB C, Pyroglyphidae immunology, Asthma therapy, Bifidobacterium breve, Hypersensitivity therapy, Inflammation therapy, Oligosaccharides pharmacology, Synbiotics administration & dosage
Abstract

Purpose: The incidence and severity of allergic asthma is rising, and novel strategies to prevent or treat this disease are needed. This study investigated the effects of different mixtures of non-digestible oligosaccharides combined with Bifidobacterium breve M-16V (BB) on the development of allergic airway inflammation in an animal model for house dust mite (HDM)-induced allergic asthma., Methods: BALB/c mice were sensitized intranasally (i.n.) with HDM and subsequently challenged (i.n.) with PBS or HDM while being fed diets containing different oligosaccharide mixtures in combination with BB or an isocaloric identical control diet. Bronchoalveolar lavage fluid (BALF) inflammatory cell influx, chemokine and cytokine concentrations in lung homogenates and supernatants of ex vivo HDM-restimulated lung cells were analyzed., Results: The HDM-induced influx of eosinophils and lymphocytes was reduced by the diet containing the short-chain and long-chain fructo-oligosaccharides and BB (FFBB). In addition to the HDM-induced cell influx, concentrations of IL-33, CCL17, CCL22, IL-6, IL-13 and IL-5 were increased in supernatants of lung homogenates or BALF and IL-4, IFN-γ and IL-10 were increased in restimulated lung cell suspensions of HDM-allergic mice. The diet containing FFBB reduced IL-6, IFN-γ, IL-4 and IL-10 concentrations, whereas the combination of galacto-oligosaccharides and long-chain fructo-oligosaccharides with BB was less potent in this model., Conclusion: These findings show that synbiotic dietary supplementation can affect respiratory allergic inflammation induced by HDM. The combination of FFBB was most effective in the prevention of HDM-induced airway inflammation in mice.

Published
2016
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2. The development of allergic inflammation in a murine house dust mite asthma model is suppressed by synbiotic mixtures of non-digestible oligosaccharides and Bifidobacterium breve M-16V

Author

Verheijden, K A T, Willemsen, L E M, Braber, S, Leusink-Muis, T, Jeurink, P V, Garssen, J, Kraneveld, A D, Folkerts, G, Pharmacology, Sub Immunopharmacology, LS Pharma, Sub Airway in vivo Pharmacology, Sub General Pharmacology, Pharmacology, Sub Immunopharmacology, LS Pharma, Sub Airway in vivo Pharmacology, and Sub General Pharmacology

Subjects
0301 basic medicine, Male, Allergy, Synbiotics, ved/biology.organism_classification_rank.species, Medicine (miscellaneous), Oligosaccharides, Bifidobacterium breve, Mice, 0302 clinical medicine, immune system diseases, Lung, Mice, Inbred BALB C, Nutrition and Dietetics, biology, Pyroglyphidae, Original Contribution, respiratory system, medicine.symptom, Bronchoalveolar Lavage Fluid, Inflammation, Allergic inflammation, House dust mite, 03 medical and health sciences, Interferon-gamma, medicine, Hypersensitivity, Animals, Asthma, Chemokine CCL22, ved/biology, business.industry, Interleukins, biology.organism_classification, medicine.disease, respiratory tract diseases, Eosinophils, Disease Models, Animal, 030104 developmental biology, Immunology, Chemokine CCL17, business, 030215 immunology
Abstract

PURPOSE: The incidence and severity of allergic asthma is rising, and novel strategies to prevent or treat this disease are needed. This study investigated the effects of different mixtures of non-digestible oligosaccharides combined with Bifidobacterium breve M-16V (BB) on the development of allergic airway inflammation in an animal model for house dust mite (HDM)-induced allergic asthma. METHODS: BALB/c mice were sensitized intranasally (i.n.) with HDM and subsequently challenged (i.n.) with PBS or HDM while being fed diets containing different oligosaccharide mixtures in combination with BB or an isocaloric identical control diet. Bronchoalveolar lavage fluid (BALF) inflammatory cell influx, chemokine and cytokine concentrations in lung homogenates and supernatants of ex vivo HDM-restimulated lung cells were analyzed. RESULTS: The HDM-induced influx of eosinophils and lymphocytes was reduced by the diet containing the short-chain and long-chain fructo-oligosaccharides and BB (FFBB). In addition to the HDM-induced cell influx, concentrations of IL-33, CCL17, CCL22, IL-6, IL-13 and IL-5 were increased in supernatants of lung homogenates or BALF and IL-4, IFN-γ and IL-10 were increased in restimulated lung cell suspensions of HDM-allergic mice. The diet containing FFBB reduced IL-6, IFN-γ, IL-4 and IL-10 concentrations, whereas the combination of galacto-oligosaccharides and long-chain fructo-oligosaccharides with BB was less potent in this model. CONCLUSION: These findings show that synbiotic dietary supplementation can affect respiratory allergic inflammation induced by HDM. The combination of FFBB was most effective in the prevention of HDM-induced airway inflammation in mice.

Published
2015

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