1. Differential Expression of Virulence and Stress Fitness Genes during Interaction betweenListeria monocytogenesandBifidobacterium longum
- Author
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Peng Li, Hengyi Xu, Tingtao Chen, Hua Wei, Qianglai Tan, Di Xu, Zoraida P. Aguilar, Xing Ming, and Feng Xu
- Subjects
DNA, Bacterial ,DNA, Complementary ,Bifidobacterium longum ,Virulence Factors ,Virulence ,Real-Time Polymerase Chain Reaction ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Biochemistry ,Analytical Chemistry ,Microbiology ,Bacterial genetics ,fluids and secretions ,Bacterial Proteins ,Listeria monocytogenes ,Stress, Physiological ,Antibiosis ,Gene expression ,medicine ,RNA, Messenger ,Molecular Biology ,Gene ,Bifidobacterium ,Microbial Viability ,biology ,Gene Expression Profiling ,Organic Chemistry ,food and beverages ,Gene Expression Regulation, Bacterial ,General Medicine ,biology.organism_classification ,Bacterial Load ,Culture Media ,RNA extraction ,Biotechnology - Abstract
Bifidobacterium is well known to have an inhibitory effect on the survival, growth, and proliferation of various foodborne pathogens, but the mechanism of the molecular action of B. longum in blocking the invasion of Listeria monocytogenes is not yet well defined. In the present study, following RNA extraction and cDNA synthesis, differential expression of virulence and stress fitness genes in L. monocytogenes and B. longum was determined by real-time PCR. The results indicate that L. monocytogenes virulence factors, including actA, hly, inlA, and plcA, showed significantly downregulated expression during co-incubation of B. longum and L. monocytogenes in phosphate-buffered saline. The relative mRNA levels of oppA and serpin, two stress fitness genes in B. longum, were significantly higher than for the control group. These results indicate that downregulation of L. monocytogenes virulence factors during co-incubation with B. longum might be responsible for the inhibitory effects.
- Published
- 2012
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