1. Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis.
- Author
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Ninomiya Y, Matsuda S, Suzuki S, Hirata-Tsuchiya S, Ueda T, Nakashima F, Yasuda K, Shimada S, Memida T, Yoshimoto T, Yamada S, Ouhara K, and Mizuno N
- Subjects
- Humans, Sp1 Transcription Factor metabolism, Down-Regulation, Cells, Cultured, Gingiva metabolism, Gingiva pathology, Adult, Protein Glutamine gamma Glutamyltransferase 2, Biglycan metabolism, Fibromatosis, Gingival metabolism, Fibromatosis, Gingival genetics, Transglutaminases metabolism, Fibroblasts metabolism, GTP-Binding Proteins metabolism
- Abstract
Background: This study aimed to elucidate the pathogenesis of idiopathic gingival fibromatosis (IGF)., Methods: Human gingival fibroblasts (hGFs) were isolated from patients with IGF and periodontitis. Differential gene expression in the hGFs was analyzed using RNA sequencing. Extracellular matrix-related gene expression in the hGFs was analyzed. The effect of specific protein (SP)1 inhibitor or recombinant human transglutaminase 2 (rh-TGM2) on biglycan (BGN) expression in hGFs was also determined., Results: RNA sequencing showed that TGM2 expression was downregulated and BGN mRNA expression was upregulated in patients with IGF relative to periodontitis. rh-TGM2 stimulation of hGFs in patients with IGF significantly reduced BGN expression. SP1 inhibitors downregulated BGN expression in the hGFs., Conclusion: BGN upregulation via SP1 causes TGM2 downregulation in gingival fibroblasts in IGF., Competing Interests: Declarations. Ethics approval and consent to participate: The study protocol was approved by the research ethics committees of Hiroshima University (E2015-0001), and written informed consent was obtained from the subjects. Consent for publication: Consent for publication was obtained from the participants. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)
- Published
- 2024
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