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Lycium barbarum L. is a species widely used in dietary supplements and natural healthcare products. The berries, also known as goji or wolfberries, mostly grow in China, but recent reports on their outstanding bioactive properties have increased their popularity and cultivation around the world. Goji berries are a remarkable source of phenolic compounds (such as phenolic acids and flavonoids), carotenoids, organic acids, carbohydrates (fructose and glucose), and vitamins (ascorbic acid). Several biological activities, such as antioxidant, antimicrobial, anti-inflammatory, prebiotic, and anticancer activities, have been associated with its consumption. Hence, goji berries were highlighted as an excellent source of functional ingredients with promising applications in food and nutraceutical fields. This review aims to summarize the phytochemical composition and biological activities, along with various industrial applications, of L. barbarum berries. Simultaneously, the valorization of goji berries by-products, with its associated economic advantages, will be emphasized and explored. [ABSTRACT FROM AUTHOR]

• Actinidia arguta is a source of bioactive compounds for food industry. • Bioactive compounds from A. arguta are reviewed. • A. arguta has high nutritional, functional and economic value. • Health promoting effects of A. arguta have been confirmed in vitro and in vivo. • A. arguta can be used in the prevention/treatment of chronic diseases (e.g. diabetes). Hardy kiwi (Actinidia arguta) is a climbing, perennial and dioecious vine from Actinidiaceae family, native from Asia and valued as ornamental and traditional medicine. In the last decade, the growing interest as fruit-bearing plant encourage the expanding cultivation of A. arguta mainly to fruits production, particularly in Europe and North America. A. arguta plants have an extensive range of bioactive compounds that can be obtained from different botanical structures, such as fruits, leaves, flowers and stems. These bioactive molecules, with well-recognized health-promoting properties, include phenolic compounds, minerals, carbohydrates or even volatile substances, with a great potential to be used in several formulations of food products. Phytochemical studies on this plant reported hypoglycemic effects as well as antioxidant and anti-inflammatory activities, among others. The traditional uses of A. arguta have been experimentally proved by in vitro and in vivo studies, in which its bioactivities were associated to its phytochemical composition. This review aims to assess and summarize the phytochemical and healthy properties of the different botanical parts of A. arguta , describing their bioactive composition and exploring it potential functional properties on foodstuffs. [ABSTRACT FROM AUTHOR]

Actinidia arguta is a climbing, fruit plant that recently generated a spread interest in many regions of the world, due to world globalization and knowledge expansion. During its production, different by-products are generated, such as fruits, leaves, stems, flowers and roots, being characterized by a rich bioactive composition that wise the scientific community for its health effects. Inboard of this panoply of valuable bioactive compounds, vitamin C, chlorogenic and neochlorogenic acids, caffeic acid, quercetin, catechin, kaempferol, p -coumaric acid, minerals and fatty acids play critical roles. As a result, the valorization and reuse of these by-products is a challenge, particularly for producers. This review aims to summarize the potential cosmetic and medical skincare applications of the different bioactive compounds from A. arguta by-products, describing and exploring their potential to contribute as active ingredients based on the biological activities reported. Simultaneously, a critical analysis of the patents available on the market regarding its use as cosmetic and medical skincare ingredient is provided. Cosmetic and medical skincare products could be a sustainable and green option for the use of A. arguta by-products bioactive compounds. Apart from contribute to skin appearance and health, the addition of these active compounds to the final cosmetic and pharmaceutical products may also provide skin beneficial effects such as photoprotection, anti-aging and anti-inflammatory effects, also acting as depigmenting and emollients, among other activities. Besides that, the impact on food by-product valorization and circular economy will be enormous. [Display omitted] • Actinia arguta by-products are rich in bioactive compounds with skin effects. •The use of A. arguta by-products in topical formulations is extremely promising. •Skin health benefits are enormous. •Few patents are available on the market with A. arguta as skin ingredient. •Add value to fruit byproducts. [ABSTRACT FROM AUTHOR]

Chayote leaves are known for culinary and traditional medicine applications. This work intended to recover carotenoids and phenolic compounds from chayote leaves using the ultrasound-assisted extraction (UAE). A Box–Behnken design was employed to investigate the impact of extraction time, temperature, and ultrasonic power on the recovery of total carotenoids, total phenolic compounds, and antioxidant activities. For comparative purposes, chayote leaf extracts were prepared by maceration (ME) and microwave-assisted extraction (MAE), using the same time and temperature conditions optimized by UAE. Extraction at 50 °C and 170 Watts for 30 min provided the optimal UAE conditions. UAE showed better extraction efficacy than ME and MAE. The HPLC analysis of the extracts showed that the xanthophyll class was the main class of carotenoids, which constituted 42–85% of the total carotenoid content, followed by β-carotene and tocopherol. Moreover, 26 compounds, classified as phenolic acids, flavonols, flavonoids and other polar compounds, were identified in the chayote leaf extracts. Flavonols accounted for 55% of the total compounds quantified (the major compound was myricetin) and phenolic acids represented around 35%, mostly represented by ferulic acid, chlorogenic acid and (+)-catechin. This study revealed the potential of UAE as an effective green extraction technique to recover bioactive compounds from chayote leaves, for food, and for pharmaceutical and cosmetic applications. [ABSTRACT FROM AUTHOR]

[Display omitted] • Lycium barbarum L. berries as a source of valuable bioactive compounds. • Response Surface Methodology enabled the optimization of the extraction process. • Phenolic acids, flavanols, and phenylamides were detected. • The extract proved to be safe for intestinal application at doses up to 500 ug/mL. • Different extract's compounds permeated the cell monolayer. Lycium barbarum L. berries have a remarkable chemical composition and extensive biological activities, being a valuable component of health and nutraceutical practices. Nevertheless, a deep insight on the intestinal permeation of the pro-healthy bioactive compounds is urgently needed to predict the real effects on human body. This study attempted, for the first time, to optimize the Ultrasound-Assisted Extraction (UAE) of goji berries using a Response Surface Methodology approach and establish the intestinal permeation of the principal pro-healthy compounds. The optimal extraction conditions were a solid:liquid ratio of 8.75 % for 56.21 min, using an intensity of 59.05 W/m2. The optimal extract displayed a remarkable antioxidant capacity, with LC/DAD-ESI-MS analysis unveiled a diverse phytochemical profile, encompassing different compounds (e.g. glu-lycibarbarspermidine F, 2-glu-kukoamine, rutin, 3,5-dicaffeoylquinic acid). The intestinal co-culture model demonstrated that glu-lycibarbarspermidine F (isomer 2) (73.70 %), 3,5-dicaffeoylquinic acid (52.66 %), and isorhamnetin-3- O -rutinoside (49.31 %) traversed the intestinal cell layer, exerting beneficial health-promoting effects. [ABSTRACT FROM AUTHOR]

Rhodophyta represents the largest seaweed phylum including a wide variety of species, such as Chondrus crispus Stackhouse, with benefits for consumers health due to their nutritional value and bioactive compounds. This work aimed to optimize the extraction of compounds from C. crispus using a three-step optimization process. The first step consisted in pigment extraction using ultrasound-assisted extraction; the performance of an ultrasonic probe (20 kHz, 750 W) and a bath (40 kHz, 120 W) for 20 or 40 min was compared. The second step corresponded to protein extraction and the third step was the carrageenan formation. Step 1 extracts contained two carotenoids (fucoxanthin and lutein), 5 chlorophyll- a derivatives and pheophythin- a , phenolic compounds and revealed moderate antiradical activity against DPPH•, ABTS•+ and O 2 •-, but no activity against •NO and cholinesterases. Proteins and carrageenan's yields were between 3.6 and 41 g/100 g and 29.7–36.1 g/100 g, respectively. Considering all the performed analyses, the use of ultrasonic bath (40 kHz, 120 W) for 40 min is efficient to extract valuable compounds from C. crispus , which is advantageous from an economic point of view and therefore more attractive for industrial purposes. The obtained extracts may find possible future application in human diet or as additives for pharmaceutic and cosmetic industry. • C. crispus bioactive compounds were extracted by a three-step optimization process. • Pigment extract revealed moderate antiradical activity against DPPH.•, ABTS•+ and O 2 •- • Pigment extract had no activity against.•NO and cholinesterases • Proteins and carrageenans yields were between 3.6% - 41% and 29.7%–36.1%. • The ultrasonic bath (40 kHz, 120 W) for 40 min was the preferable approach. [ABSTRACT FROM AUTHOR]

Highlights • Chayote possess several bioactive compounds known for their health-benefits. • Chayote possess several polyphenols compounds, vitamins and carotenoids. • The extraction of bioactive compounds from chayote is an important challenge. • In vitro and in vivo studies are vital in addressing chayote bioactivities. • Potential uses of chayote in food and non-food product development are suggested. Abstract Chayote (Sechium edule) has gained widespread consuming acceptance and recognized by its nutritional and bio-functional properties. The present review surveys and describes the current findings about the nutritional, phytochemical and pharmacological properties of chayote and identifies opportunities for further research. It also discusses chayote's versatile utility in nutrition, as a functional ingredient in food, cosmetic and pharmaceutical industries, as well as in nanotechnology and biotechnological processes. It was concluded that although the pharmacological properties of chayote are currently well-established, only a few reports have been conducted on the isolation and identification of individual chemical constituents, and similarly, only a few in vivo studies have been conducted to assess their biological efficacy. In addition, the valorisation of the underutilized chayote by-products can be an important aspect in waste management from both economic and environmental standpoints. Thus, the recovery and utilization of valuable compounds from chayote is an important challenge for scientists. [ABSTRACT FROM AUTHOR]

Since annually a high amount of wastes is produced in vine pruning, the aim of this study was to evaluate the potential of vine shoots from two Portuguese grape varieties ( Touriga Nacional - TN and Tinta Roriz - TR) to be used as a natural source of phenolic compounds. To reach this goal, three techniques were explored, namely microwave-assisted extraction (MAE), subcritical water extraction (SWE) and conventional extraction (CE). The phenolic composition of the extracts, antioxidant and biological activities were evaluated by spectrophotometry and chromatography. MAE and SWE produced the highest concentrated extracts. TR vine shoot variety had the highest antioxidant activity and total phenolic (32.1 ± 0.9 mg gallic acid equivalents/g dry sample), as well as flavonoid content (18.7 ± 1.2 mg epicatechin equivalents/g dry sample). For the first time, the biological activity of the vine shoot extracts was tested. Results demonstrated that all of them had antimicrobial potential against different bacteria and yeasts, and the ability of inhibiting α-amylase and acetylcholinesterase enzymes, with MAE TR extracts being the most efficient. HPLC analysis enabled the identification of different phenolic compounds, with gallic acid, catechin, myricetin and kaempferol-3- O -rutinoside being the main contributors to the phenolic composition. Portuguese vine shoot wastes could serve as easily accessible source of natural antioxidants for the food or pharmaceutical industries. [ABSTRACT FROM AUTHOR]

Subcritical water extraction (SWE) has become a popular green extraction technique for the isolation of different classes of compounds from natural matrices. Low price, safety and green character of water, good yields of target compounds and reduced energy consumption, make this technique favorable for potential industrial applications. The purpose of this study was to evaluate antioxidant, antimicrobial and cytotoxic activity of four medicinal plants traditionally used in folk medicine of Serbia. Black mulberry ( Morus nigra L.), wall germander ( Teucrium chamaedrys L.), wild geranium ( Geranium macrorrhizum L.) and comfrey ( Symphytum officinale L.) were extracted by subcritical water at different temperatures. Antioxidant activity of the extracts was defined by conventional spectrophotometric methods, such as the total phenolic content (TPC), DPPH-radical scavenging activity (DPPH-RSA), ferric reducing antioxidant power (FRAP) and total antioxidant capacity (TAC) assessed by a DNA-based sensor. Additionally, the main phenolic compounds contributing to the antioxidant activity of the produced extracts were also identified and quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). Antimicrobial properties of extracts were evaluated against eight microbial strains. Furthermore, the cytotoxic activity was observed for two human cancer cell lines and a cell line derived from murine fibroblast. [ABSTRACT FROM AUTHOR]

Nine plant infusions were assessed in terms of their polyphenolic compounds and antioxidant capacity properties by performing a “battery” of assays. Bioactive compounds (total phenolic and flavonoid content) were measured using spectrophotometric procedures and the total antioxidant capacity was determined by DPPH -RSA, FRAP and DNA-based biosensor against the reactive oxygen species: HO and H 2 O 2 . Although the optical methods are based on the donation of hydrogen or electrons between antioxidants and radicals, the DNA-based biosensor intends to simulate an in vivo assay by exposing DNA layer to biological ROS and antioxidants. In general, green and black tea showed higher polyphenolic content and antioxidant capacity evaluated by the described methods. All the analysed plant beverages showed to be a good source of bioactive compounds, particularly the green and black tea, peppermint and senna. [ABSTRACT FROM AUTHOR]

The effects of microwave-assisted extraction (MAE) on the qualitative and quantitative composition of extracted bioactive phytochemicals are strongly dependent on specific operational conditions. The impact of temperature, extraction time, and solvent dielectric properties on the MAE of antioxidant compounds from goji berry is hereby reported and discussed. Results were evaluated in terms of antioxidant capacity (ascertained by ABTS and ORAC assays), complemented with quantification of total phenolics content (TPC), and characterization of extracts through HPLC. Furthermore, the effect of storage temperature was also studied. Results revealed that temperature and solvent properties exert a significant effect on TPC and ORAC assays, whereas irradiation time plays a key role in the preparation of extracts to be evaluated through ABTS. The effect of solvent properties is tentatively explained considering the dielectric properties of methanol and water, whereas the observed effect of temperature could be explained by its influence on the solubility of phenolic compounds. Industrial Relevance A careful selection of operational conditions during MAE, designed for a specific antioxidant assay, allows an increase in the bioactivity of the resultant extracts, which may become important in terms of industrial applications. [ABSTRACT FROM AUTHOR]

Eight tropical fruit pulps from Brazil were simultaneously characterised in terms of their antioxidant and antimicrobial properties. Antioxidant activity was screened by DPPH radical scavenging activity (126–3987 mg TE/100 g DW) and ferric reduction activity power (368–20819 mg AAE/100 g DW), and complemented with total phenolic content (329–12466 mg GAE/100 g DW) and total flavonoid content measurements (46–672 mg EE /100 g DW), whereas antimicrobial activity was tested against the most frequently found food pathogens. Acerola and açaí presented the highest values for the antioxidant-related measurements. Direct correlations between these measurements could be observed for some of the fruits. Tamarind exhibited the broadest antimicrobial potential, having revealed growth inhibition of Pseudomonas aeruginosa . Escherichia coli , Listeria monocytogenes , Salmonella sp. and Staphylococcus aureus . Açaí and tamarind extracts presented an inverse relationship between antibacterial and antioxidant activities, and therefore, the antibacterial activity cannot be attributed (only) to phenolic compounds. [ABSTRACT FROM AUTHOR]

[Display omitted] • Shells are the most abundant chestnut by-product useful for industrial valorization. • Chestnut shells are a natural source of bioactive compounds. • Green extraction techniques are a good option to obtain high-added value compounds. • Several healthy effects have been described for bioactive compounds from shells. • Biocompounds from shells have potentialities on innovative functional foods. Castanea sativa is an outstanding species that represents a valuable natural resource for rural populations. C. sativa shells (CSS), an abundant agro-industrial by-product generated during chestnut peeling process, is commonly discarded or used as fuel. Nevertheless, CSS produced are not depleted by this application and huge amounts are still available, being particularly rich in bioactive compounds (polyphenols, vitamin E, lignin and oligosaccharides) with health benefits. Phytochemical studies reported not only antioxidant and antimicrobial activities, but also anti-inflammatory, anticancer, hypolipidemic, hypoglycemic and neuroprotective activities. The application of a suitable extraction technique is required for the isolation of bioactive compounds, being green extraction technologies outstanding for the industrial recovery of chestnut shells' bioactive compounds. CSS were highlighted as remarkable sources of functional ingredients with promising applications in food and nutraceutical fields, mainly as natural antioxidants and effective prebiotics. This review aims to summarize the phytochemical composition and pro-healthy properties of CSS, emphasizing the sustainable extraction techniques employed in the recovery of bioactive compounds and their potential applications in food and nutraceutical industries. [ABSTRACT FROM AUTHOR]

During kiwiberry production, different by-products are generated, including leaves that are removed to increase the fruit's solar exposure. The aim of this work was to extract bioactive compounds from kiwiberry leaf by employing microwave-assisted extraction (MAE). Compatible food solvents (water and ethanol) were employed. The alcoholic extract contained the highest phenolic and flavonoid contents (629.48 mg of gallic acid equivalents (GAE) per gram of plant material on dry weight (dw) (GAE/g dw) and 136.81 mg of catechin equivalents per gram of plant material on dw (CAE/g dw), respectively). Oppositely, the hydroalcoholic extract achieved the highest antioxidant activity and scavenging activity against reactive oxygen and nitrogen species (IC50 = 29.10 μg/mL for O2•−, IC50 = 1.87 μg/mL for HOCl and IC50 = 1.18 μg/mL for •NO). The phenolic profile showed the presence of caffeoylquinic acids, proanthocyanidin, and quercetin in all samples. However, caffeoylquinic acids and quercetin were detected in higher amounts in the alcoholic extract, while proanthocyanidins were prevalent in the hydroalcoholic extract. No adverse effects were observed on Caco-2 viability, while the highest concentration (1000 µg/mL) of hydroalcoholic and alcoholic extracts conducted to a decrease of HT29-MTX viability. These results highlight the MAE potentialities to extract bioactive compounds from kiwiberry leaf. [ABSTRACT FROM AUTHOR]

Wine production generates large amounts of vine-canes, a devalued by-product that could be used for the recovery of bioactive compounds. In this work, two vine-canes varieties, namely Touriga Nacional (TN) and Tinta Roriz (TR), were submitted to different ultrasound-assisted extraction (UAE) conditions. The highest phenolic and flavonoid content was observed for TR extract obtained at lab-scale without an ice bath and pilot-scale after 60 min of extraction (32.6 ± 2.1 and 26.0 ± 1.5 mg gallic acid equivalent/g dry weight (dw) and 9.5 ± 0.6 and 8.3 ± 0.8 mg epicatechin equivalents/g dw, respectively). Further, all extracts demonstrated a high antioxidant activity to scavenge DPPH free radicals with the best value reached by TR at the lab-scale without an ice bath after 30 min and pilot-scale extraction after 60 min (34.2 ± 2.4 and 33.4 ± 2.1 mg trolox equivalents/g dw, respectively). Extracts phenolic composition were also evaluated by HPLC, demonstrating that resveratrol, myricetin and catechin were the main compounds. According to our knowledge, this is the first time that a pilot scale of UAE of phenolic compounds from vine-canes was performed. This paper represents an important step to the use of UAE as an industrial process to recover bioactive compounds. [ABSTRACT FROM AUTHOR]