1. On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio
- Author
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Chia Chou Wu, Tsung Han Tsai, Mu Chien Sun, Chieh Chang, Tian Thai Lee, Yung-Jen Chuang, Che Lin, Irene Han Juo Cheng, and Bor-Sen Chen
- Subjects
Proteomics ,Cerebellum ,Critical Care and Emergency Medicine ,Microarrays ,lcsh:Medicine ,Bioinformatics ,Neurobiology of Disease and Regeneration ,Medicine and Health Sciences ,Protein Interaction Maps ,lcsh:Science ,Zebrafish ,Trauma Medicine ,Multidisciplinary ,biology ,Systems Biology ,Neurogenesis ,Fishes ,Head Injury ,medicine.anatomical_structure ,Bioassays and Physiological Analysis ,Neurology ,Osteichthyes ,Vertebrates ,medicine.symptom ,Network Analysis ,Signal Transduction ,Research Article ,Computer and Information Sciences ,Traumatic brain injury ,Models, Neurological ,Protein Array Analysis ,Brain damage ,Research and Analysis Methods ,medicine ,Animals ,Humans ,PI3K/AKT/mTOR pathway ,Wound Healing ,lcsh:R ,Organisms ,Biology and Life Sciences ,Cell Biology ,Zebrafish Proteins ,biology.organism_classification ,medicine.disease ,Signaling Networks ,Disease Models, Animal ,Brain Injuries ,lcsh:Q ,Axon guidance ,Phosphatidylinositol 3-Kinase ,Wound healing - Abstract
Upon injury, the direct damage and the subsequent secondary injury in the brain often result in chronic neurological disorders. Due to multifactorial nature of secondary injury and subsequent complex cellular responses, much of the underlying mechanisms are unclear. This study used an adult zebrafish cerebellum injury model to investigate the phenotypes and the secondary injury responses for recovery mechanisms of injured brain. Using the time course microarray analysis, a candidate protein-protein interaction (PPI) network was refined as cerebellar wound healing PPI network by dynamic modeling and big data mining. Pathway enrichment and ontological analysis were incorporated into the refined network to highlight the main molecular scheme of cerebellar wound healing. Several significant pathways, including chemokine, Phosphatidylinositide 3-kinases, and axon guidance signaling pathway and their cross-talks through PI3K, PAK2, and PLXNA3 were identified to coordinate for neurogenesis and angiogenesis, which are essential for the restoration of the injured brain. Our finding provides an insight into the molecular restoration mechanisms after traumatic brain injury, and open up new opportunity to devise the treatment for traumatic brain injury in human.
- Published
- 2014