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Your search keyword '"Doustkhah E"' showing total 2 results
Start Over Author "Doustkhah E" Topic biocatalysis
2 results on '"Doustkhah E"'

1. Covalently bonded pancreatic lipase onto the dithiocarbamate/chitosan-based magnetite: Stepwise fabrication of Fe 3 O 4 @CS/NHCS-Lip as a novel and promising nanobiocatalyst.

Author

Baghban A, Heidarizadeh M, Doustkhah E, Rostamnia S, and Rezaei PF

Subjects
Animals, Enzyme Stability, Enzymes, Immobilized chemistry, Enzymes, Immobilized metabolism, Models, Molecular, Swine, Biocatalysis, Chitosan chemistry, Lipase chemistry, Lipase metabolism, Magnetite Nanoparticles chemistry, Pancreas enzymology, Thiocarbamates chemistry
Abstract

In the present paper, porcine pancreas lipase (PPL) was immobilized to a new version of magnetite via a novel stepwise dithiocarbamate/chitosan-based method in alternation to glutaraldehyde. Magnetic chitosan nanocomposite was post-modified to produce dithiocarbamate moieties on the surface through amine functions. Then, immobilization of lipase was successfully achieved on the surface of magnetically separable Fe 3 O 4 @CS/NHCS 2 H via a post-modification. Each step of immobilization was carefully monitored by characterization and all were successfully proved. Comparison of immobilized enzyme with free enzyme showed that the method of immobilization is efficient., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Published
2017
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2. Dithiocarbamate to modify magnetic graphene oxide nanocomposite (Fe 3 O 4 -GO): A new strategy for covalent enzyme (lipase) immobilization to fabrication a new nanobiocatalyst for enzymatic hydrolysis of PNPD.

Author

Heidarizadeh M, Doustkhah E, Rostamnia S, Rezaei PF, Harzevili FD, and Zeynizadeh B

Subjects
Animals, Enzymes, Immobilized chemistry, Enzymes, Immobilized metabolism, Hydrolysis, Models, Molecular, Nanocomposites chemistry, Oxides chemistry, Protein Conformation, Swine, Biocatalysis, Graphite chemistry, Laurates metabolism, Lipase chemistry, Lipase metabolism, Magnetite Nanoparticles chemistry, Nitrobenzenes metabolism, Thiocarbamates chemistry
Abstract

Immobilization of lipase was successfully achieved on the surface of magnetically separable Fe 3 O 4 /graphene oxide (GO) via a post-modification. This post modification was achieved in alternation to glutaraldehyde post-modification. The activity of immobilized lipase had not a significant loss in the activity while on the other hand, it is simply extractable (by keeping its major activity) from reaction crude by a magnet. Each step of immobilization was carefully monitored by characterization and all were successfully proved. SEM, TEM, XRD, EDX, and FTIR were used to characterize the support and immobilization process., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Published
2017
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