1. Highly efficient single molecule detection in microstructures.
- Author
-
Dörre K, Stephan J, Lapczyna M, Stuke M, Dunkel H, and Eigen M
- Subjects
- Image Processing, Computer-Assisted, Polymethyl Methacrylate, Spectrometry, Fluorescence instrumentation, Spectrometry, Fluorescence methods, Biochemistry instrumentation, Biochemistry methods, Fluorescent Dyes analysis
- Abstract
For DNA single molecule sequencing, the complete detection of all dye-labeled monomers which are cleaved off during the sequencing reaction is an essential requirement. In this work we address the feasibility of single molecule detection in microstructures with a confocal multi element set-up. We present statistical data on single molecule recognition and explain a refined data evaluation technique for single molecule burst analysis. From these data the signal-to-noise ratio in microstructures is evaluated as well as the overall detection efficiency. So far, detection efficiencies of single molecule events of up to 60% have been shown in microstructures.
- Published
- 2001
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