7 results on '"Eman Maher Zahran"'
Search Results
2. Anti-epileptic potential, metabolic profiling and in silico studies of the aqueous fraction from Ocimum menthiifolium benth, family Lamiaceae
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Samar Yehia Desoukey, M. Alaraby Salem, Usama Ramadan Abdelmohsen, Mohamed Kamel, Ahmed Kolkeila, Hany Ezzat Khalil, Mostafa A. Fouad, and Eman Maher Zahran
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Family Lamiaceae ,Aqueous solution ,Biochemistry ,biology ,Chemistry ,In silico ,Organic Chemistry ,Plant Science ,Ocimum ,biology.organism_classification ,Analytical Chemistry - Abstract
The current study aimed to investigate the anti-epileptic potential of the ethanol extract and its different fractions from the Lamiaceous plant, Ocimum menthiifolium. The results revealed that the aqueous fraction with the latest onset of myoclonic convulsions (1095 ± 45**** s) was the most biologically active one. This was followed by LC-HR-MS-coupled metabolic profiling which led to dereplication of 8 compounds from that fraction. A molecular docking study was performed on the dereplicated compounds to discover the main responsible ones for the activity. The results highlighted Apigenin-7,4'-di-O-glucoside as the top scoring ligand with a possible mechanism of action involving the modulation of the voltage-gated sodium channel.
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- 2020
3. Scabicidal Potential of Coconut Seed Extract in Rabbits via Downregulating Inflammatory/Immune Cross Talk: A Comprehensive Phytochemical/GC-MS and In Silico Proof
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Eman Maher Zahran, Nehad M. Reda Abdel-Maqsoud, Omar. Y. Tammam, Islam M. Abdel-Rahman, Mahmoud A. Elrehany, Hussain T. Bakhsh, Faisal H. Altemani, Naseh A. Algehainy, Mubarak A. Alzubaidi, Usama Ramadan Abdelmohsen, and Abeer H. Elmaidomy
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Microbiology (medical) ,Infectious Diseases ,coconut seed extract ,Sarcoptes scabiei ,scabies ,GC-MS ,IL-1β ,IL-6 ,IL-10 ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics ,Biochemistry ,Microbiology - Abstract
Scabies is an invasive skin condition caused by Sarcoptes scabiei mites. The present study investigates the antiscabies potential of coconut seed extract (CSE) in rabbits. GC-MS analysis of the seed oil identified 17 known compounds, while CSE phytochemical investigation afforded 4 known ones. The topical application of seed extract improved all signs of infection, and the improvement started 3 days post application. However, in vitro application of the extract caused 99% mortality of mites 1 day post application. Histopathological examination revealed the absence of inflammatory infiltration and hyperkeratosis of the epidermis, compared with ivermectin-treated groups which revealed less improvement. The mRNA gene expression results revealed a suppression of IL-1β, IL-6, IL-10, MMP-9, VEGF, and MCP-1, and an upregulation of I-CAM-1, KGF as well as TIMP-1. The docking analysis emphasized a strong binding of gondoic acid with IL-1β, IL-6, and VEGF with high binding scores of −5.817, −5.291, and −8.362 kcal/mol, respectively, and a high binding affinity of 3″(1‴-O-β-D-glucopyranosyl)-sucrose with GST with −7.24 kcal/mol. Accordingly, and for the first time, our results highlighted the scabicidal potential of coconut seed extract, which opens the gate for an efficient, cost-effective as well as herbal-based alternative for the control of scabies in rabbits.
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- 2022
4. Antioxidant and Wound Healing Potential of Vitis vinifera Seeds Supported by Phytochemical Characterization and Docking Studies
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Tarfah Al-Warhi, Eman Maher Zahran, Samy Selim, Mohammad M. Al-Sanea, Mohammed M. Ghoneim, Sherif A. Maher, Yaser A. Mostafa, Faisal Alsenani, Mahmoud A. Elrehany, Mohammed S. Almuhayawi, Soad K. Al Jaouni, Usama Ramadan Abdelmohsen, and Abeer H. Elmaidomy
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Physiology ,Clinical Biochemistry ,food and beverages ,Cell Biology ,Vitis vinifera ,benzofuran ,wound healing ,TNF-α ,TGF-β ,in silico ,Molecular Biology ,Biochemistry - Abstract
This study explored the in vivo wound healing potential of Vitis vinifera seed extract using an excision wound model with focus on wound healing molecular targets including TGFBR1, VEGF, TNF-α, and IL-1β. The wound healing results revealed that V. vinifera seed extract enhanced wound closure rates (p < 0.001), elevated TGF-β and VEGF levels, and significantly downregulated TNF-α and IL-1β levels in comparison to the Mebo®-treated group. The phenotypical results were supported by biochemical and histopathological findings. Phytochemical investigation yielded a total of 36 compounds including twenty-seven compounds (1–27) identified from seed oil using GC-MS analysis, along with nine isolated compounds. Among the isolated compounds, one new benzofuran dimer (28) along with eight known ones (29–36) were identified. The structure of new compound was elucidated utilizing 1D/2D NMR, with HRESIMS analyses. Moreover, molecular docking experiments were performed to elucidate the molecular targets (TNF-α, TGFBR1, and IL-1β) of the observed wound healing activity. Additionally, the in vitro antioxidant activity of V. vinifera seed extract along with two isolated compounds (ursolic acid 34, and β-sitosterol-3-O-glucopyranoside 36) were explored. Our study highlights the potential of V. vinifera seed extract in wound repair uncovering the most probable mechanisms of action using in silico analysis.
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- 2022
5. Multitarget in silico studies of Ocimum menthiifolium, family Lamiaceae against SARS-CoV-2 supported by molecular dynamics simulation
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Tarfah Al-Warhi, Narek Abelyan, Mostafa A. Fouad, Usama Ramadan Abdelmohsen, Amgad Albohy, Samar Yehia Desoukey, M. Alaraby Salem, Hany Ezzat Khalil, Eman Maher Zahran, Mohamed Kamel, and Mohammad M. Al-Sanea
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biology ,Chemistry ,In silico ,fungi ,Virulence ,General Medicine ,Ocimum ,biology.organism_classification ,Virus ,Prunin ,chemistry.chemical_compound ,Metabolomics ,Biochemistry ,Docking (molecular) ,Structural Biology ,Lamiaceae ,Molecular Biology - Abstract
The novel strain of human coronavirus, emerged in December 2019, which has been designated as SARS-CoV-2, causes a severe acute respiratory syndrome. Since then, it has arisen as a serious threat to the world public health. Since no approved vaccines or drugs has been found to efficiently stop the virulent spread of the virus, progressive inquiries targeting these viruses are urgently needed, especially those from plant sources. Metabolic profiling using LC-HR-ESI-MS of the butanol extract of Ocimum menthiifolium (Lamiaceae) aerial parts yielded 10 compounds including flavonoids, iridoids and phenolics. As it has been previously reported that some flavonoids can be used as anti-SARS drugs by targeting SARS-CoV-1 3CLpro, we chose to examine 14 flavonoids (detected by metabolomics and other compounds isolated via several chromatographic techniques). We investigated their potential binding interactions with the 4 main SARS-CoV-2 targets: Mpro, nsp16/nsp10 complex, ACE2-PD and RBD-S-protein via molecular docking. Docking results indicated that the nsp16/nsp10 complex has the best binding affinities where the strongest binding was detected with apigenin-7-O-rutinoside, prunin and acaciin with −9.4, −9.3 and −9.3 kcal/mol binding energy, respectively, compared to the control (SAM) with −8.2 kcal/mol. Furthermore, the stability of these complexes was studied using molecular dynamics of 150 ns, which were then compared to their complexes in the other three targets. MM-PBSA calculations suggested the high stability of acaciin-nsp16 complex with binding energy of −110 kJ/mol. This study sheds light on the structure-based design of natural flavonoids as anti-SARS-CoV-2 drugs targeting the nsp16/10 complex. Communicated by Ramaswamy H. Sarma
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- 2020
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6. Local anaesthetic potential, metabolic profiling, molecular docking and in silico ADME studies of Ocimum forskolei, family Lamiaceae
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Samar Yehia Desoukey, Eman Maher Zahran, Markus Krischke, Hany Ezzat Khalil, Mostafa A. Fouad, Usama Ramadan Abdelmohsen, Mohamed Kamel, M. Alaraby Salem, Martin J. Mueller, and Mahmoud M Shalash
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Family Lamiaceae ,Local anaesthetic ,biology ,010405 organic chemistry ,In silico ,Organic Chemistry ,Ethyl acetate ,Plant Science ,Ocimum ,biology.organism_classification ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Metabolomic profiling ,chemistry ,ADME - Abstract
The present study aimed to detect the bioactive metabolites from Ocimum forskolei aerial parts which are responsible for the local anaesthetic activity of the ethyl acetate fraction. Following a bioassay-guided fractionation, twelve compounds were dereplicated from the ethyl acetate fraction which was the most potent one with a mean onset of action (1.43 ± 0.07****) min compared to tetracaine as a positive control (1.37 ± 0.07****) min. These compounds, along with seven other compounds (isolated by diverse chromatographic techniques) were subjected to a molecular docking study to declare the top scoring compounds predicted to be responsible for such activity. The results highlighted Rabdosiin and Apigenin-7-O-rutinoside as the main bioactive leaders of the local anaesthesia via forming multiple H- bonding with the sodium ion channels leading to their blockade and loss of pain sensation, which strongly supports the use of O. forskolei as a local anaesthetic agent.
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- 2020
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7. Antiulcer potential and molecular docking of flavonoids from Ocimum forskolei Benth., family Lamiaceae
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Samar Yehia Desoukey, A Samir Hussein, Hany Ezzat Khalil, M. Alaraby Salem, Mostafa A. Fouad, Eman Maher Zahran, Usama Ramadan Abdelmohsen, and Mohamed Kamel
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Ethanol ,biology ,Traditional medicine ,010405 organic chemistry ,Organic Chemistry ,Ethyl acetate ,Plant Science ,Fractionation ,biology.organism_classification ,Ulcer index ,Ocimum ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,chemistry ,Lamiaceae ,Petroleum ether ,Dichloromethane - Abstract
The present study aimed to detect the bioactive metabolites from Ocimum forskolei aerial parts which are responsible for the antiulcer activity of the total ethanol extract (TEE) as well as different fractions (petroleum ether, dichloromethane, ethyl acetate and aqueous). Six flavonoids were isolated from the dichloromethane fraction which was the most potent; with an ulcer index value of 2.67 ± 2.18*** and % inhibition of ulcer of 97.7%; following a bioassay-guided fractionation. The isolated flavonoids were subjected to molecular docking analysis in an attempt to explain their significant antiulcer potential, and the results revealed that salvitin followed by sideritiflavone were the main active ones acting against M3 and H-2 receptors, respectively. Moreover, a molecular dynamics simulation illustrated the formation of two persistent H-bonds between salvitin and the two amino acids of the active site (Asn507 and Asp147) formed in 42 and 65% of the frames, respectively.
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- 2019
- Full Text
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